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Interventional Cardiology Journal
ISSN: 2471-8157
2
n d
E d i t i o n o f E u r o S c i C o n C o n g r e s s o n
Heart Disease and
Interventional Cardiology
F e b r u a r y 2 5 - 2 6 , 2 0 1 9
P a r i s , F r a n c e
Heart and World Cardiology 2019
Background:
Salviamiltiorrhiza (SM) is a Chinese herbwidely used for ischemic
heart diseases (IHD), yet little is known about the cellular mechanisms. The
aims of this study were to investigate mechanisms of SM.
Methods:
The rat A10 cells line, a vascular smooth muscle cells line isolated
from rat thoracic embryonic aorta was as a study model. The SM roots aqueous
extract , MTT assay, cytotoxicity assay, two-dimensional electrophoresis
coupled with MALDI-TOF mass spectrometry , western blot analysis, and
biological network analysis were applied for the elucidation of protein changes
characterizing the response of the rat A10 cells into the homocysteine(Hcy)
-induced oxidative stress.
Results:
Our study showed that a low dose (0.015 mg/mL) of the SM
significantly inhibited growth (>60%, p < 0.05) of the Hcy stimulated rat A10
cells. In addition, concentration of intracellular reactive oxygen species
obviously decreased in the rat A10 cells after its incubation with SM in terms
of catalase increasing activity. Next, marked down-regulation of protein kinase
C beta-1 and phosphorylated mitogen-activated protein kinase expression
suggest that observed inhibitory effect of the SM on the Hcy-induced growth
of rat A10 cells was realized via the PKC/p44/42 MAPK- dependent pathway.
The intensity changes of 10 protein spots in response of the rat A10 cells to
the Hcy-induced oxidative damage as alpha-4-tropomyosin, vimentin, F1F0-
ATP synthase (beta subunit), glucose regulated protein 75, actin (fragment),
prohibitin, capping protein, plakoglobin, endoplasmic reticulum protein 29,
and peptidylprolyl isomerase A, were detected with statistical significance (p
< 0.05). Meanwhile, it was showed that used here SM resist carbonylation of
vimentin, alpha-4-tropomyosin and GRP75, respectively, leading to phenotype
transformations in the rat A10 cells.
Conclusion:
These data suggest that SM may exert its protective effect in IHD
through circulating ROS suppression and subsequent modulation of protein
carbonylation in rat aortic smooth muscle cells.
Biography
Yu-Chiang Hung grew up in Taiwan and received his Bachelor's
degree from the China Medical University in 1990 , another
M.D . degree from the National Yang-Ming University in 1992
, and Ph.D. degree from the Chang Gung University in 2010.
His PhD thesis was “Functional proteomic study in bioactive
compounds of radix Salvia miltiorrhiza”. Dr. Hung had found
that Salvia miltiorrhiza could inhibit vascular smooth muscle
cell proliferation to treat atherosclerosis. He is an attending
and director of Department of Chinese medicine in Kaohsiung
Chang Gung Memorial Hospital now. His specialty is Chinese
medicine , cardiovascular diseases ,Chinese herbs , and
acupuncture. He has published 39 papers in SCI journals and
has been serving as an editorial board member of repute.
hungyuchiang@gmail.comAntioxidative effects of complementary therapy with Salvia
miltiorrhiza in ischemic heart disease
Yu-Chiang Hung
Department of Chinese Medicine, Kaohsiung Chang Gung Memorial Hospital , Taiwan
Yu-Chiang Hung, Interv Cardiol J 2019, Volume: 5
DOI: 10.21767/2471-8157-C1-005