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E u r o p e a n C o n g r e s s o n
Vaccines & Vaccination
and Gynecologic Oncology
Vaccines & Vaccination and Gynecologic Oncology 2018
O c t o b e r 2 6 - 2 7 , 2 0 1 8
B u d a p e s t , H u n g a r y
Critical Care Obstetrics and Gynecology
ISSN: 2471-9803
Biography
Charulata has completed her PhD and she is senior consultant
embryologist at Yashoda Fertility and Research Institute,
Hyderabad, India. She has a 18 long years’ experience in field
of ART. She keeps herself updated with recent advances
in ART and regular in writing Abstracts, Posters, Oral paper
presentations in national and international forum.
To evaluate the relation of delayed channel dissolution in
oocytes after ICSI with embryo quality and pregnancy rates
Charulata Chatterjee and Papolu Rama Devi
Yashoda Fertility and Research Institute, India
Dr. Rama's Institute for Fertility, India
Objective: To evaluate the relation of delayed channel dissolution in oocytes after
intracytoplasmic sperm injection (ICSI) with embryo quality and pregnancy rates
Design: Randomized study
Patient(s):Total of 62 embryo transfers for couples undergoing IVF-ICSI procedure
Main outcome measure(s): Channel formation and timing of oocyte restoration
after ICSI procedure, pronuclear, cleavage stage and pregnancy rate observation.
Methods: Out of 208 oocytes, 196 metaphase II oocytes were ICS injected.
According to oocyte responses like channel shape and timing of restoration after
ICSI, division was made in two groups. Group 1 is positive channel formation and
normal restoration immediately after ICSI. Group 2 is delayed channel dissolution
after ICSI (±2mins).
Results: Out of 196 injectedoocytes in127oocytes channel dissolved immediately
whereas in 69 oocytes it was delayed restoration of channel. Fertilization rate
between Group 1 and 2 were 84.92%, 68.25% respectively. ≥40% fragmentation
is observed in group 2 (20.45%). 9 transfers on day 3 were with the embryos
formed by constant channel. Out of 2 pregnancies (22.2%), one resulted in
miscarriage. Remaining 53 embryo transfers were from the embryos derived from
ICSI procedure where oocytes gained spherical shape immediately after ICSI. 20
(37.73%) resulted positive for pregnancy (p=0.3718).
Conclusion: This small group study suggests oocytes with viscous cytoplasm
may result in aneuploid embryos, which may be a reason for miscarriage. Careful
observation can help to select good quality embryo.
Charulata Chatterjee et al., Crit Care Obst & Gyne 2018, Volume: 4
DOI:10.21767/2471-9803-C1-002