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Food Chemistry 2018 Journal of Food, Nutrition and Population Health
ISSN: 2577-0586 Page 82
July 23-24, 2018
Rome, Italy
3rd Edition of International Conference on
Agriculture &
Food Chemistry
Industrial bioethanol production generates huge amounts of
wastewater from distillation process called stillage which
is fractionated into liquid (thin stillage) and solid (condensed
distillers grains) fractions. Thin stillage contains all of the
non-volatile components of the grain not utilized by the yeast
(oligosaccharides, lipids, protein) as well as their metabolism by-
products (i.e. glycerol, organic acids) and is, in part, recycled in
the process (called backset). Those could be utilized by edible
filamentous fungi to produce food/feed biomass, moreover
application of edible yeast strain capable of fast utilization of
inhibitory compounds (lactate, glycerol) may improve removal
of some compounds which may accumulate and inhibit the
fermentation. In this study, industrial grade corn-based thin
stillage (9%w/v dry solids) was used as medium for production of
edible Aspergillus oryzae in co-culture with fodder yeast Candida
utilis inoculated after initial 24 h of fungal growth. The 72-h co-
culture resulted in obtaining up to 18.6 g L-1 biomass with crude
protein and lipid content of 0.31 and 0.41 g g-1 respectively also
containing all of the essential amino acids. Therefore, the biomass
could be used as a high protein and energy, vegan foodstuff or
animal feed additive. Additionally, the cultivation resulted in
reduction of 33.9% of thin stillage solids, 33.4% glycerol and
12.5% lactic acid. Moreover, additional 4.5 g L-1 ethanol was
produced together with high amount of amylolytic enzymes (1.67
U mL-1) which could be sent back to the process and positively
contribute to its course and final production economics.
Biography
Dr. Witold Pietrzak has completed his MSc and PhD in Food Technology
by Wroclaw University of Environmental and Life Sciences. He works as
assistant at Department of Fermentation and Cereals Technology. He has
published 12 papers in reputed journals.
Edible biomass production by co-culture of Aspergillus oryzae
and Candida utilis using corn-based thin stillage
Witold Pietrzak, Joanna Kawa Rygielska
Wroclaw University of Environmental and Life Sciences, Poland
Witold Pietrzak et al., J Food Nutr Popul Health 2018, Volume 2
DOI: 10.21767/2577-0586-C2-006