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Volume 3, Issue 2 (Suppl)
Trends in Green chem
ISSN: 2471-9889
Environmental & Green Chemistry 2017
July 24-26, 2017
Page 122
5
th
International Conference on
6
th
International Conference on
July 24-26, 2017 Rome, Italy
Environmental Chemistry and Engineering
Green Chemistry and Technology
&
Codisplay of diisopropyl-fluorophosphatase (dfpase) and organophosphorus acid anhydrolase (opaa)
enzymes in cell surface of e.coli
Samaneh Khodi, Ali Mohammad Latifi,
Baqiyatallah University of Medical Sciences, Tehran, Iran.
Backgrounds:
Pesticides used in controlling harmful populations of insects, can adversely affect human health and cause
environmental pollution. The most popular pesticides are organophosphates (OPs) such as Diazinon, Chlorpyrifos, Malathion,
etc. Several enzymes have been isolated able to degrade different kinds of OPs such as OPAA (from cytoplasm of
Alteromonas
sp.) and DFPase (from the brain of squid
Loligo vulgaris
). To reduce the OP uptake limitation and degrade a broader substrate
spectrum with more activity, we demonstrate for the first time a functional codisplay of two distinct hydrolases, DFPase and
OPAA, on a single bacterial cell, by fusing each enzyme to N-terminal of InaV (InaV-N).
Materials and Methods:
To cotranslocate, target genes should be cloned in two different plasmids with two different antibiotic
resistances and independent expression strategy, therefore,
inaV-N/dfpase and ina
V-N/
opaa
fragments were cloned into pET-
28a(+) and pCDFDuet vectors, separately. Competent E. coli Bl21 cells were transformed by individual recombinant plasmids,
as controls, and simultaneously both one by electroporation method.
Results:
Studies on activity by FPLC with DFPase, OPAA and codisplay cells showed a specific activity of 19.676, 13.125 and
22.46 U/ml, respectively, for Diazinon, and 10.779, 5.213 and 13.784 U/ml, respectively, for Chlorypyrifos. Study on activity by
monitoring fluoride-release from DFP by each miligram of DFPase, OPAA and codisplay wet cells showed 500, 250 and 800
Unit, respectively.
Discussion:
Findings showed a recombinant strain possesses a broader substrate spectrum and a more activity than strains
expressing either one of the hydrolases, a whole-cell biocatalyst candidate to detoxify environment polluted by different OPs.
Khodi.sama@gmail.comElectrochemical reactor design/fabrication for CO
2
electroreduction to low-carbon fuels/chemicals
Yuyu Liu
1
and
Jinli Qiao
2
1
Shanghai University, China
2
Donghua University, China
E
xtra emission of carbon dioxide (CO
2
) into the atmosphere, induced by human industrial activities, has been considered
one of the primary causes in possible global warming due to the greenhouse effect, and also becoming an increasing
concern in recent years. To address this issue, using electrochemical reduction to convert CO
2
to useful small fuels such as
CH
4
, CH
3
OH, HCOOH, C
2
H
2
, etc., represents a value-added approach to the simultaneous generation of alternative fuels
and environmental remediation of carbon emissions from the continued use of conventional fuels. Many references in the
literature have focused on studies with improving the performance of electrochemical process to convert CO
2
into low-carbon
fuels/chemicals. For reactor components, both anodes and cathodes are discussed in terms of their materials, structure,
design, fabrication, as well as their effects on the reactor’s performance and productivity. Several important electrodes are
described including metallic electrodes, gas diffusion electrodes (GDEs) and modified metal electrodes in both aqueous and
non-aqueous electrolyte solutions. Both the ion-exchange membranes for low-temperature operation, and the solid oxide
electrolyte layers for high-temperature operation, can serve as both functions of ion conduction and electrode separating.
Regarding the electrochemical reactor design/assembling/fabrication for CO
2
reduction, several types of the reactors, including
the conventional three-electrode cells, the low temperature two-electrode cells using proton exchange membrane fuel cell
(PEMFC) technology, the solid oxide electrolysis cells (SOECs) and the microbial electrolysis cells (MECs), as well as other
electrolysis cells, are introduced and their performances are also analyzed. The potential and feasibility of the reactor scale-up
for CO
2
conversion to low-carbon fuels/chemicals is discussed with respect to the technology’s commercialization.
liuyuyu@shu.edu.cnTrends in Green chem, 3:2
DOI: 10.21767/2471-9889-C1-003