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Biochemistry & Molecular Biology Journal
ISSN: 2471-8084
Internat i ona l Conference on
Biotechnology, Biomarkers
& Systems Biology
M a r c h 0 4 - 0 5 , 2 0 1 9
Am s t e r d a m , N e t h e r l a n d s
Biotechnology, Biomarkers & Systems Biology 2019
O
steoarthritis knee is one of the most prevalent orthopaedic disorders
worldwide. In Indian subcontinent, the prevalence of this disorder
is estimated to be in the range of 22% to 39%. Many studies have been
conducted for confirmation of association of genetic factors with clinical
and radiological features of osteoarthritis knee. Nearly all have proven role
especially in association with CALM-1, ESR-alpha, GDF-5 gene etc. Still we
are diagnosing such a majorly prevalent disorder on the basis of clinical and
radiological criteria only. We conducted a case control study in between for
confirmation of the same and found statistically significant results. 120 cases
and 120 controls were enrolled. Clinical and radiological features were noted
and clinical scoring was done using VAS, WOMAC and Lequesne’s scoring
system. Genetic polymorphism in relation to intronic region of CALM 1 gene
was studied by DNA extraction, PCR and RFLP method. Statistical analysis
was done using Stata software. CALM-1 gene intronic SNP (rs3213718) was
found in significantly higher number in cases than controls (p value=.0022).
Logistic regression has also proved significant association of occurrence of
Single nucleotide polymorphism (SNP) with disease. We concluded that CALM
1 gene intronic SNP (rs3213718) is present in Indian Population. The target
SNP significantly affects the disease as the difference between cases and
controls is highly significant (p value=.0022). We further discussed that there
have been multiple such publications confirming the role of genetic factors
in causation and progression of osteoarthritis knee. These criteria should be
incorporated as biomarkers in diagnosis of osteoarthritis knee.
Biography
Sachin Avasthi is an Associate Professor and Head in
Department of Orthopaedic Surgery, DR RML Institute of
Medical Sciences, Lucknow. His qualifications are MBBS,
MS, PhD. He has an experience of 19 years in the field of
Orthopaedic Surgery after completing his graduation. He has
more than 120 publications to his credit which include scientific
papers, abstracts and book chapters. He has achieved ISAKOS
scholarship which is a prestigious association of sports injury.
He is an achiever of Health Icon Award by the State Government
of UP in India. He has guided more than 45 students in their
Postgraduate thesis work. He is Member of many national and
international associations such as AO Trauma, IOA etc.
sachinavasthi4778@gmail.comIncorporation of genetic markers in diagnostic criteria of
osteoarthritis knee
Sachin Avasthi
RML Institute of Medical Sciences, India
Sachin Avasthi, Biochem Mol biol J 2019, Volume:5
DOI: 10.21767/2471-8084-C1-023