biotechnology-biomarkers-systems-biology-2019-posters

Page 63

Biochemistry & Molecular Biology Journal

ISSN: 2471-8084

Internat i ona l Conference on

Biotechnology, Biomarkers

& Systems Biology

M a r c h 0 4 - 0 5 , 2 0 1 9

Am s t e r d a m , N e t h e r l a n d s

Biotechnology, Biomarkers & Systems Biology 2019

everal experimental and clinical studies report that

Hypericum perforatum

L. (HP) can be effective in treating various disorders

related to the central nervous system, such as depression and substance use disorder. Cancer is known as a disease that not

only impairs life and physical integrity, but also affects mental health. This study aimed at investigating the anticancer activity of

the HP extract in an

in vitro

model of esophageal squamous cell carcinoma (ESCC) (i.e., KYSE-30 cells) and its interference with

Adriamycin (Adr) as a conventional chemotherapeutic drug. To this aim, cancerous and normal cells (5000 cells/mL) were exposed

to both anticancer agents in triplicate to be subsequently tested using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium

bromide (MTT) assay. A quantitative real-time polymerase chain reaction (PCR) assay was performed to evaluate the mRNA

gene expression of Cyclin D1. Both treatments reduced the cell count in a dose-dependent fashion. The IC

of Adr and HP was

~0.090-0.095 mg/mL and ~0.92-0.94 mg/mL in KYSE-30 cells. The maximum inhibition of KYSE-30 cell was 55.9% and 57.1% by

individual Adr and HP, respectively. Combined treatments of KYSE-30 cells with Adr and HP significantly reduced the cell viability

and yet elevated the level of cyclin D1 expression as opposed to the individual treatments. The presence of HP was found to

decrease Adr concentrations below the therapeutic range. The co-treatment of cancer cells with HP led to poor response to Adr

and accordingly multidrug resistance as verified by notable up-regulation of cyclin D1 gene expression. Thus, the simultaneous

use of Adr and HP is not recommended in cancer disease.

The potential interaction between adriamycin

and Hypericum perforatum L

Issa Amjadi

, Hamid Reza Fathi

, Motahare-Sadat Hosseini

Wayne State University, USA

Tehran University of Medical Sciences, Iran

Psychiatry and Behavioural Sciences Research Center-Mashhad University of Medical Sciences,

Iran

Biochem Mol biol J 2019, Volume:5

DOI: 10.21767/2471-8084-C1-024