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Volume 10
Journal of Archives of Medicine
Advanced Biotechnology & Annual Pediatrics 2018
November 28-29, 2018
Page 16
Novel Trends and Advances in Biotechnology,
Cell & Stem Cell Research
15
th
Annual Congress on Pediatrics
World Congress on
&
November 28-29, 2018 Barcelona, Spain
Joint Event On
Akinneye J O
Federal University of Technology Akure, Nigeria
Isolation and characterization of
Eugenia aromatica
oil extract against tropical warehouse moth
Ephestia
cautella
[Lepidoptera: pyralidae] in cocoa beans
C
ocoa bean is a raw material used for the production of chocolate and other confectionaries.
Ephestia cautella
is the major
pest of dried cocoa beans in storage and synthetic insecticide like organo-chlorides and organophosphates are the major
insecticides used to control this pest in storage which further post health hazard toman and his environment.This then necessitate
the search for insecticide of plant origins which are bio-degradable and non-toxic to man. This study investigates the contact
and fumigant efficacy of the powder and oil extract of
Ephestia aromatica
on the developmental stages of
E. cautella
. Powders
of
E. aromatica
were administered at different concentrations (0.5 g, 1.0 g, 1.5 g, 2.0 g and 2.5 g). The oil from
E. aromatica
was extracted with ethanol using soxhlet extractor and redistilled using rotary evaporator and tested as fumigant insecticidal
against development stages of
E. cautella
at 0.5 ml, 1.0 ml, 1.5 ml, 2.0 ml and 2.5 ml. Egg hatchability, adult emergence, larvae
and adult mortality of
E. cautella
were used as indices of insecticidal activities at 24 hrs, 48 hrs, 72 hrs and 96 hrs post-treatment.
Essential oil obtained from the plant was purified using thin layer chromatography and analyzed by Gas Chromatography Mass
Spectrometer (GC-MS). Result obtained shown that
E. aromatica
powder and oil completely inhibited egg hatchability and adult
emergence both as contact and fumigant. Except the 0.5 g of
E. aromatica
powder that recorded 50.00% larva mortality and
51.67% adult mortality, other treatment concentrations recorded 90-100% larva and adult mortality. At 2.5 ml oil extract tested
as contact and fumigant larvicides after 96 hrs recorded 92.98% and 98.23% mortality, respectively. Results from phytochemical
analysis of the oil showed that the major components were eugenol (82.044%) and Caryophyllene (11.716%). These findings
suggested that
E aromatica
extract could be a potential source of insecticide which may be used for the production of bio-
pesticide.
Biography
Akinneye J O is an Associate Professor and has his expertise in the field of applied entomology and in the evaluation of medicinal plant for the control of stored
product pest. He has provided new strategies for local farmers for the control of stored product pests. His research interest is focused on the biology and control of
lepidopterous pest of stored products. He has 15 years of research, teaching and administration at the Federal University of Technology Akure, Ondo State, Nigeria,
where he has supervised many undergraduate and postgraduate students.
josephakinneye@yahoo.co.ukAkinneye J O, Arch Med 2018, Volume 10
DOI: 10.21767/1989-5216-C2-004