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Volume 8, Issue 6 J Neurol Neurosci
ISSN: 2171-6625 Neuro, an open access journal Neuroscience 2017
October 16-17, 2017
OCTOBER 16-17, 2017 OSAKA, JAPAN
17TH Global Neuroscience Conference
Learning deficit and altered MMP9 and TIMP1 gene expression in adult rats exposed to bacterial endotoxin
during early postnatal development
Alexander Nikolaevich Trofimov1,2, Alexander Schwarz1, Kevin Fomalont1,3, Viktoria Schukina1, Ekaterina Veniaminova1,5, Nataliia Markova1,2,5, Olga
Zubareva1,4 and Victor Klimenko1
1Institute of Experimental Medicine, Russia
2University Hospital of Wuerzburg, Germany
3National Institute of Allergy and Infectious Diseases, USA
4I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russia
5Institute of General Pathology and Pathophysiology, Russia
Perinatal brain pathologies are known to impair the development of CNS functioning and are involved in the etiology of
chronic cognitive dysfunction. These pathological conditions are associated with high production of pro-inflammatory
cytokines by the cells of the immune and nervous systems. It is well established that neurons express receptors for pro-
inflammatory cytokines, which provides evidence for the functioning of cytokines as neuromodulators. However, the exact
molecular and cellular mechanisms of cytokines in the impairment of brain development have not yet been fully elucidated.
To address this question, we studied the expression of neuroplasticity-regulating genes matrix metalloproteinase-9 (MMP9)
and tissue inhibitor of metalloproteinases-1 (TIMP1) in the medial prefrontal cortex and dorsal and ventral hippocampus.
Wistar rat pups were treated with lipopolysaccharide (LPS; 25 μg/kg), an inducer of pro-inflammatory cytokine synthesis,
during the 3rd week of postnatal life. Adolescent and adult LPS-treated animals demonstrated increased anxiety-like behavior
and decreased exploratory behavior in the open field arena. Impaired learning in the active avoidance task and Morris water
maze was also observed. Gene expression of MMP9 and TIMP1 was differentially altered in the cortex and hippocampus
of pups vs. adult untrained rats and remained unchanged in rats trained in either learning task, revealing that prolonged
pro-inflammatory challenge during early postnatal development negatively affects the plasticity factors involved in memory
acquisition in adulthood. These results suggest that an increase in cognitive stimulation might be an effective approach to
reduce the negative effects of neonatal immune challenges on brain functioning.
Biography
Alexander Nikolaevich Trofimov is a Research Fellow and pursuing his PhD at the Institute of Experimental Medicine, Saint Petersburg, Russia. He has completed
his BSc and MSc degrees in 2009 and 2011, respectively from Saint Petersburg State University, Russia. His work focuses on the investigation of molecular and
cellular mechanisms in the context of immune activation in CNS functioning and in impaired brain development.
Alexander Nikolaevich Trofimov et al., J Neurol Neurosci 2017, 8:6
DOI: 10.21767/2171-6625-C1-006