neurology-2018-posters-abstracts

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4 t h E u r o S c i C o n C o n f e r e n c e o n
Neurology & Neurological
Disorders
Neurology 2018
J u l y 1 2 - 1 3 , 2 0 1 8
P a r i s , F r a n c e
Page 66
Journal of Neurology and Neuroscience
ISSN: 2171-6625
The study focuses on the degenerations of peripheral and central
nervous system at relevant disease phases in mice affected with
SOD, an animal model for Amyotrophic Lateral Sclerosis (ALS), using
X-ray phase-contrast tomography (XPCT). Compared to standard
X-ray Tomography, XPCT is an advanced technique that allows three-
dimensional reconstruction of bio-medical samples without any
sectioning or aggressive preparation or use of contrast agents. XPCT
is a powerful technique to analyze low absorbing objects and enables a
multiscale imaging ranging fromcellular-level up to
analyzed mice spinal cords at different stages of ALS, providing deeper
knowledge on the degeneration of motor neurons and vascularization
in the central nervous system as well as their 3D spatial distribution.
The analysis was therefore extended to the peripheral nervous system,
both in the anterior and posterior spinal nerves, as the peripheral
motor nerve damage precedes neuronal degeneration within the spinal
cord. We will show, at different time points, the quantification of the
variations in the vascular and neuronal networks of the spinal cord,
already detectable in a pre-symptomatic stage of the disease. We
correlated these results with those obtained in the peripheral nervous
system, where, thanks to the high spatial resolution, we quantify the
orientation of spinal nerve fibers. This preclinical study will be able to
lay the groundwork for future clinical applications
X-ray phase contrast tomography for the investigation of
ALS disease
G Begani Provinciali1 , M Fratini 1,2, I Bukreeva1 , F Palermo1, N
Pieroni1 , L Maugeri2 ,G Gigli1 , A Quattrini3 and A Cedola1
1Institute of Nanotechnology, Consiglio Nazionale delle Ricerche (CNR-NANO), Roma, Italy
2IRCCS Santa Lucia Foundation, Rome, Italy
3Institute Of Experimental Neurology (INSPE), Ospedale San Raffaele, Italy
G Begani Provinciali et al., J Neurol Neurosci 2018, Volume: 9
DOI: 10.21767/2171-6625-C1-009
Biography
G Begani Provinciali has completed her Master’s degree in January
2018 and she is employed as a Research Fellow at the Institute of
Nanotechnology (CNR, Rome Unit). She has published one paper in
reputed journals and she is Co-author of a book chapter in press.