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E u r o S c i C o n C o n f e r e n c e o n
Neurology & Neurological
Disorders
Neurology 2018
J u l y 1 2 - 1 3 , 2 0 1 8
P a r i s , F r a n c e
Page 64
Journal of Neurology and Neuroscience
ISSN: 2171-6625
C
enters for Advancing Research on Botanicals and Other Natural
Products (CARBON) Icahn School of Medicine at Mount Sinai,
Supported by P50 AT008661 Center Grant by NCCIH and ODS Dietary
polyphenols have been investigated for their role in promoting
memory in model systems of stress, but little is known about select
subpopulations of neurons that are influenced by polyphenols to
improve memory performance. Granule neurons in the hippocampal
dentate gyrus are vulnerable to stressors that impair the functioning
of contextual memory and can be influenced by dietary polyphenols.
We utilized a c-fos-tTA/TRE-ChR2 optogenetics model in which
neurons activated during fear learning are labelled with ChR2-mCherry
and can be optically reactivated in a different context to recapitulate
the behavioural output of a related memory. Treatment with dietary
polyphenols increased fear memory recall and ChR2-mCherry
expression in dentate gyrus neurons in the same animal, suggesting
that dietary polyphenols promote recruitment of neurons to a fear
memory engram. We show that dietary polyphenols promote memory
function and offer a general method to map cellular subpopulations
influenced by dietary polyphenols, in part through the mechanism of
c-Fos expression enhancement.
Dietary polyphenols enhance optogenetic recall of fear
memory in hippocampal dentate gyrus granule neuron
subpopulations
J Brathwaite, C Smith, T Frolinger, J Wang and G M Pasinetti
Icahn School of Medicine at Mount Sinai, USA
Biography
Justin Brathwaite has graduated from Columbia University in 2014 with a
Bachelors of Arts, BA in Chemistry. Since graduation, he has been working
full time as an Associate Researcher at the Icahn School of Medicine,
Center of Molecular and Integrative Neuro-resilience directed by Dr. Giulio
Pasinetti MD/PhD, under a Research Supplement to promote diversity in
health related research awarded by the National Institute of health (NIH).
Jbrath812@gmail.comJ Brathwaite et al., J Neurol Neurosci 2018, Volume: 9
DOI: 10.21767/2171-6625-C1-009