Electronic Journal of Biology

AHA SFRN Postdoctoral Research Fellow, Columbia University Medical Center, New York, New York, USA

Dr. Riddhi Shah currently working as an American Heart Association postdoctoral research fellow at Columbia University Medical Center, New York. She is currently expanding her knowledge in the field of sleep and cardiovascular disease. she has expertise in the field of Molecular Biology and Genetics in the past 8 years and she is now expertise in Sleep Restriction and its effect on the endothelial cells. For four years she worked as teaching assistant in Department of Biological Sciences, at Louisiana Tech University.

Genetics and Molecular Biology techniques: Molecular Cloning, Recombinant DNA technology, DNA Ligation, Expression Clone construction, DNA Shuffling, PCR techniques, RT PCR, PCR based mutagenesis, Site-directed Mutagenesis, DNA Sequencing, Analyzing DNA sequence for random and site-specific mutation, TALEN construction (Golden Gate), TAL DNA binding domain, CRISPR/ Cas9, Recombinant protein expression, Isolation of nucleic acids (plasmid and genomic DNA), DNA Sequencing, RNA Isolation, mRNA Capping and Tailing, Agarose gel electrophoresis, Southern Blotting, Western Blotting, Immunoblotting, DNA fragmentation, Elution of nucleic acids, DNA Quantification, DNA Gel Extraction, DNA purification using columns, Blood WBC Isolation, EPC Isolation, Platelet Activation Cell Culture Techniques: In vitro study, Gene transfer studies like Transfection of Eukaryotic cells using Transfection reagent, Electroporation, Passaging the mammalian cells, Picking colonies from cell culture plates, Antibiotic Selection and Culturing of cells, Cell culture stock preparation, Hypoxia Experiments on HUVEC cell line. Microbial techniques: Isolation and Identification of Bacteria, Antibiotic Sensitivity testing, Bacterial competent cell preparation, Liquid Culture Transformation, Bacterial Transformation, Bacterial culture maintenance and glycerol stock preparation, Yeast Transformation, Yeast Competent Cell preparation, Yeast Stock preparation, Yeast Genomic Extraction Biochemical techniques: LbL self-assembly, Langmuir- Blodgett films, Biochemical identification of microorganisms, Estimation of sugars, proteins, lipids and nucleic acids, Chromatographic techniques, Cytotoxicity (MTT) assay, Lysosomal phosphatase assay, Nitric oxide assay Microscopy: Florescence Microscope, Flow Cytometry