15 / 20
Volume 4
Clinical Pediatric Dermatology
ISSN: 2472-0143
Page 67
JOINT EVENT
Wound Congress 2018 &
Clinical Dermatology Congress 20
18
October 15-16, 2018
October 15-16, 2018 Rome, Italy
&
5
th
International Conference on
Advances in Skin, Wound Care and Tissue Science
14
th
International Conference on
Clinical Dermatology
To develop an
in vivo
wound healing model for human skin
Xunwei Wu
1
, Jie Wen
1
, Xue Leng
1
and
Qian Zhou
1
1
Shandong University, China
T
he repair and management of full-thickness skin wounds such as deep burns or chronic ulcers remain a clinical challenge.
No significant new therapies have been made in the last decade due in large part to the absence of a properly humanized
animal model. Currently, the common mouse model for studying human skin wound healing uses either human skin grafted
or tissue engineering skin transplanted onto the nude mice. In the human skin graft model, the skin transplant after wounding
usually repairs with contraction, a high rate of shrinkage and hypertrophic scar, and does not maintain a viable normal-
looking human skin for long periods. The current tissue engineering skin model is not morphologically normal as it consists
of a simple bilayer epidermis without appendages and an undifferentiated dermis. We recently developed a new isolation and
culture system, which could maintain multipotential of skin cells after expanded
in vitro
. The culture-expanded skin cells
were able to regenerate a full-thickness human reconstituted skin (hRSK) after grafting onto the immuodeficient mouse. The
histological structure of hRSK is similar to that of human skin and we created a wound on the regenerated skin, and found the
skin could heal by itself, and the healing procedure is similar to normal human skin. This data suggested this mouse model
could used to study human skin wound healing
in vivo
.
Clin Pediatr Dermatol 2018, Volume 4
DOI: 10.21767/2472-0143-C2-006