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Volume 4
Clinical Pediatric Dermatology
ISSN: 2472-0143
Page 66
JOINT EVENT
Wound Congress 2018 &
Clinical Dermatology Congress 20
18
October 15-16, 2018
October 15-16, 2018 Rome, Italy
&
5
th
International Conference on
Advances in Skin, Wound Care and Tissue Science
14
th
International Conference on
Clinical Dermatology
Novel wound assessment using
in vitro
wound models with mass spectrometry imaging
E. E. L. Lewis
Innovenn UK Ltd, Uk
Aim:
The normal wound healing process is a highly ordered and structured process involving overlapping four main phases:
haemostasis, inflammation, proliferation and remodelling. Damage to the skin’s integrity, which is caused trauma or injury
initiates the wound healing process. Infections are known to disrupt the normal wound healing and have a detrimental effect
on this process, which has shown to induce chronic wound formation. The western world is currently facing a public health
crisis due to the increasing number of people suffering from chronic wounds. Identifying biomarkers associated with the
wound healing process may aid early detection of a wound developing into an infected or chronic wound. The use of Labskin,
a living skin equivalent model to imitate non-infected and infected wounds will provide the opportunity to assess wound
healing process in-depth. Combining Labskin with Mass Spectrometry Imaging (MSI) would allow simultaneous multi-
analyte detection
in situ
to help identify specific markers linked to wound healing.
Methods& Results:
Labskin was wounded with scalpel blade, which was either non-infected or immediately infected with S.
aureus and left for up to 4 days. Samples were snapped frozen, sectioned at wound site, coated inMALDI matrix and analysed for
lipids (mass range: 400-1200 m/z) whereas, histology samples were stained with haematoxylin and eosin. Principal component
analysis (PCA) was performed on regions of interest (dermis, epidermis and wound site/infection). MS/MS profiling and
database search was able to putatively identify ions of interest in the non-infected sample where a glycosylceramide was shown
to be present in the epidermis across the wound site.
Conclusions:
The wounded Labskin model with MSI was able to putatively identify a glycosylceramide within the wound site
and epidermis, which may be used a biomarker associated with the wound healing process.
Clin Pediatr Dermatol 2018, Volume 4
DOI: 10.21767/2472-0143-C2-006