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May 24-25, 2018
London, UK
Vascular Surgery 2018
3
rd
Edition of World Congress & Exhibition on
Vascular Surgery
Journal of Vascular and Endovascular Therapy
ISSN: 2573-4482
Statement of the Problem:
Nuclear factor (erythroid-derived 2)-
like 2 (NRF2) is a global antioxidant gene inducer whose activity
may regulate metabolism of extracellular matrix proteins
including collagen. It was shown that human abdominal aortic
aneurysm has increased deposition of collagen I and reduced
of collagen III in tunica media and adventitia. The synthesis of
collagen I is controlled by transforming growth factor beta 1
(TGFb1). Therefore, the purpose of this study was to describe
localization of structural collagens within the aorta and aortic
aneurysm in transcriptional knockouts of Nrf2 and to verify the
mechanism behind those changes.
Methodology & Theoretical Orientation:
We used a model of
angiotensin II (Ang II)-induced abdominal aortic aneurysm in
old adult mice (6 mo.) with transcriptional knockout of Nrf2
(Nrf2 -/-) and with normal activity of Nrf2 (Nrf2 +/+). Mice
were administrated with Ang II (1000 ng/kg/min) or saline
(sham group) for 28 days via osmotic minipumps placed
subcutaneously. After 28 days tissue specimens were collected
for immunofluorescence and analysis of gene expression.
Findings:
Ang II-treatment caused a significant increase of
collagen I mRNA expression in tunica adventitia and a strong
increase of collagen III expression in tunicamedia. The observed
upregulation of collagen type I and III was significantly higher in
mice lacking transcriptional Nrf2. An increase in collagens was
associated with significantly higher TGFb1 only in the Nrf2 -/-
mice.
Conclusion & Significance:
Transcriptional factor Nrf2 may
play a significant role in collagen deposition during abdominal
aortic aneurysm formation and excessive collagen synthesis
may be associated with TGFb1 activation in the Nrf2-/- mice.
Figure 1:
Altred localisation of collagen I and collagen III within the aortic
aneurysm of transcriptional knockouts of Nrf2 mice. L-lumen.
Recent Publications
1. Loboda (2017) Role of Nrf2/HO-1 system in
development, oxidative stress response and diseases:
an evolutionarily conserved mechanism. Cellular and
Molecular Life Sciences 73(17):3221-47.
2. Rodella (2016) Abdominal aortic aneurysm and
histological, clinical, radiological correlation. Acta
Histochemica 118(3):256-62.
Lack of transcriptional activity of
Nrf2
affects
TGFb1
expression and alters collagen I and III localization within mice
aortic aneurysm
Aleksandra Piechota Polanczyk
1
, Ewa Werner
1, 2
, Karolina Hajduk
1
, Aleksandra
Kopacz
1
, Damian Kloska
1
, Anna Grochot Przeczek
1
, Ihor Huk
3
and
Alicja Jozko-
wicz
1
1
Jagiellonian University, Poland
2
University of Agriculture in Krakow, Poland
3
Medical University of Vienna, Austria
Aleksandra Piechota Polanczyk et al., J Vasc Endovasc Therapy 2018, Volume 3
DOI: 10.21767/2573-4482-C1-002