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Structural Biology 2018
Volume: 4
Biochemistry & Molecular Biology Journal
Page 74
March 15-16 2018
Barcelona, Spain
10
th
Edition of International Conference on
Structural Biology
D
-mannose is a natural sugar present in various foods, and
binds to
E. coli,
which is then discharged through urine. The
purpose of this research is to prove the efficiency of D-mannose
and provide an approach to the mean duration of treatment by
performing tests on laboratory rats, by provocation of urinary
tract infection (UTI). This was done by infecting the rats by
different ways and then administration of D-mannose orally. A
bacteriological examination of urine was carried out and the
interpretation of results was based on the sterility of the culture
media. Another aim of this research is the study of protein-
protein interactions, which have an important role to understand
the process of pathogenesis of bacterial and viral infections.
Bioinformatics analysis contributes to the study of protein-
protein interactions with the help of softwares for molecular
dynamics and protein-protein docking. We have studied the
interaction between D-mannose and the FimH protein by using
molecular dynamics. Initially, several structural calculations
and optimizations by Hyperchem8 software were conducted
on D-mannose to understand how the natural sugar attacks the
E. coli
bacterium. Then docking calculations were performed
by Hex6.3. Interpretation of results is based on the energy of
interaction formed by ligands
α
-D-mannose and β-D-mannose.
The lowest energy of interaction of complex probably presents a
greater inhibition of FimH protein.
nezhataleb@yahoo.frA docking moleculal calculations to study the properties of
the D-mannose as a natural treatment of urinary infections
caused by E. coli
Nezha Sekkal-Taleb
Djillali Liabes University, Algeria
Biochem Mol biol J, Volume 4
DOI: 10.21767/2471-8084-C1-009