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Mass Spectrometry 2019
March 04-05, 2019
Berlin, Germany
Mass Spectrometry
9th Edition of International Conference on
International Journal of Drug Development and Research
ISSN: 0975-9344 Page 22
Models of chromatography separation and their
application for improving SEC resolution
Milos Netopilik
Institute of Macromolecular Chemistry, Czech Republic
The effectivity of chromatography separation,
i.e., resolution depends on construction of the
chromatograph in the first place on the detectors cells
volume and for polymer of given molecular weight,
strongly on operational variables, as concentration and
flow-rate. The effect of concentration is not important for
polymers in theta solvents. However, the concentration
effect is important for polymers in. The effective
hydrodynamic volume of dissolved macromolecules
decreases with increasing concentration. The spatial
distribution of the analyte with respect to the longitudinal
axis of the separation system, developing in time, can
be approximated by the binomial distribution and the
elution curve is obtained numerically from the spatial
distributions developing in time. The elution curve
is then obtained by observing the developing spatial
distribution in a fixed point representing the detectors.
The concentration effect is observed as deviations from
this model which can be calculated numerically.
Biography
Milos Netopilik has completed his PhD at Institute of Macro-
molecular Chemistry and Postdoctoral studies from Virginia
Polytechnic Institute and Technical University. Now, he works
in Institute of Macromolecular Chemistry, Academy of Scienc-
es of the Czech Republic andworks in the theory of separation.
He has published more than 68 papers in reputed journals.
Milos Netopilik, Int J Drug Dev & Res 2019, Volume 11
DOI: 10.21767/0975-9344-C1-005