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Infectious Diseases
and STD-AIDS
Infectious Diseases and STD-AIDS 2018
Journal of Transmitted Diseases and Immunity
ISSN 2471-8084
A p r i l 2 6 , 2 7 2 0 1 8
R o m e , I t a l y
Page 70
J Transm Dis Immun 2018 Volume 2
DOI: 10.21767/2573-0320-C1-003
W
ith the worsening trends of drug resistance, there is a need for newer and more powerful antimicrobial agents. The search
for new compounds originating from natural resources is a promising research area. We hypothesized that animals living
in polluted environments are potential source of novel antimicrobial molecules. Under polluted milieus, organisms such as
cockroaches encounter different types of microbes, including superbugs. Such creatures survive the onslaught of superbugs
and are able to ward off diseases by producing antimicrobial substances. Here, we characterized antibacterial properties in
extracts of various body organs of cockroaches (
Periplaneta americana
) and showed potent antibacterial activity in crude brain
extract against methicillin-resistant
Staphylococcus aureus
and neuropathogenic E. coli K1. The size-exclusion spin columns
revealed that the active compound(s) are less than 10 kDa in molecular mass. Using cytotoxicity assays, it was observed that
pre-treatment of bacteria with lysates inhibited bacteria-mediated host cell cytotoxicity. Using spectra obtained with LC-MS on
Agilent 1290 infinity liquid chromatograph, coupled with an Agilent 6460 triple quadruple mass spectrometer, tissues lysates
were analysed. Among hundreds of compounds, only a few homologous compounds were identified that contained isoquinoline
group, chromene derivatives, thiazine groups, imidazoles, pyrrole containing analogs, sulfonamides, furanones, flavanones,
and known to possess broad-spectrum antimicrobial properties, and possess anti-inflammatory, anti-tumour, and analgesic
properties. Further identification, characterization and functional studies using individual compounds can act as a breakthrough
in developing novel therapeutics against various pathogens including superbugs.
naveed5438@gmail.comWar on terror cells: novel sources of antimicro-
bials
Naveed Ahmed Khan
1
, Salwa Mansur Ali
1
, Ayaz Anwar
2
, Muham-
mad Raza Shah
2
, Peter Heard
1
and Ruqaiyyah Siddiqui
1
1
Sunway University, Malaysia
2
HEJ Research Institute-University of Karachi, Pakistan