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Infectious Diseases 2018

Journal of Prevention and Infection Control

ISSN: 2471-9668

Page 67

June 07-08, 2018

London, UK

8

th

Edition of International Conference on

Infectious Diseases

E

nterobacter cloacae

bacterium is a known symbiont of most

Anopheles

gutmicroflora and nominated as a proper candidate

for paratransgenic control of malaria. Here, we describe a strategy

that uses symbiotic bacteria to deliver anti-malaria effector

molecule to the midgut lumen, thus rendering host mosquitoes

refractory to malaria infection.

Enterobacter cloacae

was

engineered to secret defensin, a natural plant anti-Plasmodium

effector protein. The engineered

E. cloacae

inhibited oocyst

formation of the rodent malaria parasite

Plasmodium berghei

by

up to 92.8% in

Anopheles stephensi

. Significantly, the proportion

of mosquitoes carrying parasites (prevalence) decreased by up to

75%. Interestingly, the wild strain of E.cloacae could inhibit oocyst

formation by up to 72%. These findings provide the foundation for

the use of either wild or geneticallymodified

E.cloacae

bacteria as

a powerful tool to combat malaria.

moshaghi@sina.tums.ac.ir

Fighting malaria using paratransgenic vectors using engineered

Enterobacter cloacae expressing defensin

Mohammad Ali Oshaghi, Hossein Dehghan

and

Seyed Hassan Moosa Kazemi

Tehran University of Medical Sciences, Iran

J Prev Infect Cntrol 2018, Volume 4

DOI: 10.21767/2471-8084-C1-003