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Immunology 2018
J u l y 0 5 - 0 7 , 2 0 1 8
V i e n n a , A u s t r i a
Page 65
Journal of Clinical Immunology and Allergy
ISSN 2471-304X
1 5 t h I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology
Chemoattractant complement 5a receptor (C5aR) is associated with
mucosal immunity. Although C5aR is expressed in several cell types
including macrophages, monocytes, and neutrophils, its expression
is controversial in mucosal dendritic cell (DC) subsets. In this study,
we found that CD11c+ CD11b-CD8- Peyer’s patch (PP) DCs located in
subepithelial dome expressed the C5aR where stimulation of C5aR
with its cognate ligand, C5a, or a specific peptide (Co1) effectively
induced antigen-specific IFN-γ+ Th1 cells through the induction of pro-
inflammatory cytokines. Based on the previous observation that Co1
peptide has an M cell-targeting ability and its moiety is homologous
to C5aR agonist, EP67, which is capable of inducing CD8+ cells, we
assumed that oral administration of Co1 peptide-conjugated antigen
may induce the antigen-specific Th1 and/or CD8 response through
both M cell antigen-targeting and the induction of pro-inflammatory
cytokines via C5aR signalling in systemic and mucosa immune
compartments. To this end, a model antigen, partial-nonstructural 3
(NS3) protein of dengue virus serotype 2 (DENV-2) was conjugated
with Co1 peptide (p-NS3-Co1) and M cell-targeting of the antigen and
co-localization with C5aR on M cells were confirmed. As we assumed,
oral prime and boost immunization with p-NS3-Co1 effectively induced
the NS3-specific IFN-γ + effector CD8+ T cells. In addition, challenge with
DENV-2 at 4 weeks post immunization with p-NS3-Co1 induced not
only the functional restimulation of memory effector CD8+ T cells but
also proliferation of CD107a+ cytolytic effector CD8+ T cells in mucosal
and systemic compartments. Collectively, we concluded that C5aR
plays a role as mucosal immune modulator in PPs and Co1 peptide
ligand-mediated C5aR activation contributes to develop the CD8+ T cell
immune response induction.
C5a receptor signalling in Peyer’s patch dendritic cells
enhances the antigen-specific CD8+ T cell response in dengue
mucosal vaccine model
Sae-Hae Kim1,2, Jisang Park2 and Yong-Suk Jang1,2
Dept. of Molecular Biology, Chonbuk National University, Jeonju 561-756, Korea
Biography
Yong-Suk Jang has completed his PhD from Northwestern University,
Chicago, IL, USA in 1993 and Postdoctoral study at Cancer Research
Institute of Seoul National University Medical School, Seoul Korea. He is a
Professor and Chairman in the Department of Molecular Biology, College
of Natural Science, Chonbuk National University, Republic of Korea. He has
published more than 150 papers in reputed journals including the Journal
of Immunology, Biomaterials, European Journal of Immunology, and
Scientific Reports. He will serve as a President of the Korean Association
of Immunologists for 2019. His current research is mainly on Mucosal
Immune Regulation and Mucosal Vaccine Development.
Yong-Suk Jang et al., Insights Allergy Asthma Bronchitis 2018, Volume: 4
DOI: 10.21767/2471-304X-C1-003