3 / 3
Biochemistry & Molecular Biology Journal
ISSN: 2471-8084
Page 23
Biotechnology, Biomarkers & Systems Biology
March 04-05, 2019 | Amsterdam, Netherlands
International Conference on
Biotechnology, Biomarkers & Systems Biology 2019
B
iomarker study on dementia has developed and most reliable fluid
markers are amyloid peptide (Aβ), TAUand phosphorylatedTAUdetected
in cerebrospinal fluid. In addition, there is great interest in blood-based
markers of Alzheimer’s disease (AD) since blood extraction is much less
invasive. Moreover, plasma biomarkers can be measured at relatively low
expense once a standard system of measurement is established. However,
there is not yet an established or validated diagnostic test for plasma
biomarkers. Using a neuronal cell culture model we have found that annexin
A5 andMilk fat globule-EGF factor 8 protein (MFG-E8), Ca
2+
and phospholipid
binding proteins were elevated in the cell culturemediumby Aβ42 treatment.
Immunohistochemical study using AD mouse model (APPPS1) brains
revealed characteristic distributions of annexin A5 and MFG-E8: more
intensive staining with anti-annexin A5 antibody was observed widely in
APPPS1 mice compared with control; whereas staining with anti-MFG-E8
antibody was detected only in the central part of the anti-Aβ-antibody stained
plaque in APPPS1 mice, while no-staining was observed in control. As both
annexin A5 and MFG-E8 might cross the blood brain barrier due to their lipid
bindingproperty, it isplausible that bothproteinsmight beplasmabiomarkers
for AD. For measuring plasma levels of them, we established ELISA systems
with monoclonal antibodies against annexin A5 and MFG-E8, respectively.
The concentrations of both annexin A5 andMFG-E8 were significantly higher
in AD patients than in the healthy individuals (P<0.0001). From the ROC curve
with plasma annexin A5 and MFG-E8 concentrations for the AD/control, the
mean areas under the curve were 0.898 and 0.723, respectively. Interestingly,
the level of plasma annexin A5 was also significantly higher in MCI patients
than in control (P<0.0001). This suggest that annexin A5 was elevated an
early stage of the onset of AD.
Biography
Hitoshi Sohma has completed his PhD in
BiochemistryatHokkaidoUniversity,Japan,focusing
on Ca
2+
signalling in cell-cell communications, and
his Postdoctoral studies at the National Institute
of Mental Health, NIH, USA. He is now a Professor
in the Department of Educational Development,
Sapporo Medical University Center for Medical
Education, Sapporo, Japan. He is involved in both
patho-biochemical research and the management
of medical education at the university. Recently, he
has engaged in community medical care program
through inter-professional education. His research
interest is dementia-related pathophysiology of Ca
2+
signalling. The results of this research should prove
to be of value in community health care education.
sohma@sapmed.ac.jpHitoshi Sohma et al., Biochem Mol biol J 2019, Volume:5
DOI: 10.21767/2471-8084-C1-022
Annexin A5 and MFG-E8 as potential plasma
biomarkers for Alzheimer's disease
Hitoshi Sohma, Ayaka Sudo
and Yasuo Kokai
Sapporo Medical University Center for Medical
Education, Japan