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Volume 3, Issue 4 (Suppl)
Polym Sci
ISSN: 2471-9935 Polym Sci, an open access journal
October 12-13, 2017 Osaka, Japan
Annual Meeting on
Biopolymers and Drug Delivery Systems
Biopolymers Meeting 2017
October 12-13 2017
Page 45
Novel and validated spectrophotometric estimation of Indomethacin in bulk and solid dosage formulation
using mixed hydrotropic solubilization technique
Nisar Ahmad Khan and Jameela Rasool
University of Kashmir, India
A
round 40%of NewChemical Entities (NCE’s) are poorlywater soluble and not well absorbed after oral administration. To overcome
the problems associated with oral absorption and bioavailability of these poorly soluble drugs, various strategies have been utilized
out of which hydrotropic solubilization has shown promising results. The present study was undertaken on Indomethacin-a water
insoluble drug to enhance its solubility using mixed hydrotropic solubilization technique followed by development and validation of
UV spectrophotometric method for quantitative estimation of Indomethacin in bulk and pharmaceutical dosage forms. Preliminary
solubility studies were carried out with different molars of hydrotropic agents and from the solubility studies performed, proper
blend (1.5 M Sodium citrate and 1.5 M Sodium benzoate) of hydrotropic agents was selected for further study. UV spectrum and
calibration curve of Indomethacin was established in selected blend of hydrotropic agents and it was found to exhibit λmax of 320
nm. Analysis of bulk drug of Indomethacin and marketed samples in chosen blend of hydrotropic agents was done and total drug
content was calculated. The developed method was then validated as per ICH guidelines for linearity and range, accuracy, precision,
limit of detection (LOD) and limit of quantification (LOQ). There was significant increase in solubility of Indomethacin with increase
in molarity of hydrotropic agents and a blend of 1.5 M Sodium citrate and 1.5 M Sodium benzoate showed good results. Thus, it is
concluded that the mixed hydrotropic solubilization can be effectively used for solubility enhancement of poorly water-soluble drugs
and the proposed UV method which is new, simple, accurate and reproducible can be successfully employed for the routine analysis
of Indomethacin in bulk samples as well as other pharmaceutical dosage forms.
Biography
Nisar Ahmad Khan is presently working as Senior Assistant Professor in the Department of Pharmaceutical Sciences, University of Kashmir, India. He has obtained
his Bachelor’s degree in Pharmacy from University of Kashmir and Master’s degree in Pharmaceutics from SGSITS Indore, India. He has obtained his Doctorate
degree in Pharmaceutics from University of Kashmir in the field of novel drug delivery systems. He has his expertise in formulation of hydrodynamically balanced
drug delivery systems in the form of single unit or multiple units and has expertise in the Gastroretentive DRUG delivery systems (GRDDS). He has also worked in
the field of solid dispersion technology, hydrotropic solubilization and cosmetology.
nakhan2008@gmail.comNisar Ahmad Khan et al., Polym Sci 2017, 3:4
DOI: 10.4172/2471-9935-C1-006