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Volume 3, Issue 4 (Suppl)
Polym Sci
ISSN: 2471-9935 Polym Sci, an open access journal
October 12-13, 2017 Osaka, Japan
Annual Meeting on
Biopolymers and Drug Delivery Systems
Biopolymers Meeting 2017
October 12-13 2017
Page 43
Comparison of the bio stimulating capacity of degradation of Poly(3-hydroxybutyrate) by forage plants
and microorganisms in simulated soil
Matheus Marques Torres, Mariane Igansi Alves, Karine Laste Macagnan, Camila Rios Piecha, Patrícia Diaz de Oliveira, Claire Tondo Vendruscolo and Angelita
da Silveira Moreira
Federal University of Pelotas, Brazil
P
olyhydroxybutyrate [P(3HB)] is a microbial polyester and possess characteristics adequate to petrochemical plastics substitution
as polypropylene. It is completely biodegradable and the speed of degradation depends on environmental characteristics as
microbiota, temperature and humidity. So, we measured the degradation rate of P(3HB) synthesized by the bacterium
Ralstonia
solanacearum
[P(3HB) RS] in a simulated soil model.The aimof the study was to evaluate the degradation capability effectiveness of the
bacteria
Ralstonia solanacearum
and
Bacillus megaterium
CN3 and the bacterial degradation bio stimulating capability of the foraging
plants
Lolium multiflorum
(Ryegrass) and
Lotus corniculatus
(Birdsfoot trefoil). With P(3HB) RS, produced by bacterium
Ralstonia
solanacearum
RS and commercial P(3HB) Biocycle® [PHB Industrial S.A., Brazil] (control), films were produced by solubilizing up 1g
in 40 mL of chloroform for 30 min at 58 °C and evaporation in petry plate to film formation. Samples were cut, weighed and separated
into polyester bags with three samples each and buried in the soil to be removed at intervals of 20, 40, 60, 80 and 100 days. Trays for
plant germination, containing individual cells full of commercial organic soil were used. The soil treatments were: (1) Natural soil
grown with both plants, (2) natural soil grown with ryegrass, (3) natural soil grown with Birdsfoot trefoil, (4) natural soil without
plants, (5) sterilized soil, (6) sterilized soil inoculated with R.
solanacearum
, (7) sterilized soil inoculated with B.
megaterium
, and
(8) only natural soil. The plants used did not stimulate the biodegradation, but despite that, they helped in the fragmentation of the
sample. For bacterial treatments, it was possible to affirm that B.
megaterium
is a more effective polymer degrader. Moreover, we can
attest that polymer degradation is more effective in a microbiologically more favorable environment, since the treatment of unsterile
soil without plants (trat.8) obtained the highest rate of degradation (100%).
Biography
Matheus Marques Torres is currently pursuing Bachelor’s degree in Biotechnology at the Federal University of Pelotas, Brazil. He has developed his research activities
in the Laboratory of Biopolymers where he works with studies related to the production, characterization and biodegradation of the bioplastic polyhydroxybutyrate
(P(3HB).
matheus_mmt@hotmail.comMatheus Marques Torres et al., Polym Sci 2017, 3:4
DOI: 10.4172/2471-9935-C1-006