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Aquaculture 2018
S e p t e m b e r 2 0 - 2 1 , 2 0 1 8
L i s b o n , P o r t u g a l
Page 31
Journal of
ISSN: 1307-234X
E u r o p e a n S u mm i t o n
Aquaculture, Fisheries
and Horticulture
Albert K D Imsland et al., Journal of Volume:12
DOI: 10.21767/1307-234X-C1-003
Lumpfish Cyclopterus lumpus is a commercially important fish in several
areas of its range in the North Atlantic Ocean. This species also plays a
vital role in salmonid aquaculture where it serves as a biological agent of
sea lice control. Recent investigations into the cleaning behaviour (sea lice
grazing efficacy) and disease resistance in several families of lumpfish
showed significant difference among families, of which, the genetic basis is
yet to be investigated for selective breeding programmes. Despite the fishery
and aquaculture importance of lumpfish, few genetic resources are currently
available for the species. Here, a genomic approach with different next-
generation sequencing platforms was used to gain insight into the patterns
of genome-wide variation in a wild cohort (n=30) of lumpfish distributed
along the Norwegian coastline. First, this study assembled a draft genome
of lumpfish using two differently size-selected, from Illumina paired-end read
libraries (MiSeq) and from a 400 bp single-end read library using the Ion Torrent
sequencing platform. Second, single nucleotide polymorphisms (SNPs) and
small structural variants (insert-deletions, INDELS; or complex variants) were
isolated and characterized using a double digest restriction associate DNA
sequencing (ddRAD) approach. Finally, the complete mitochondrial genome
was assembled and annotated for lumpfish and its phylogenetic placement
within the order Scorpaeniformes was determined. The extensive genomic
information reported here will facilitate molecular ecology studies and many
aspects of the selective breeding programme of lumpfish, especially for
marker-assisted selection.
Biography
Albert K D Imsland finished his PhD degree in Aquaculture
from University of Bergen, Norway in 1997 and has been an
Adjunct Professor from this University since 2004. He is the
Research Director in Aquaculture in the Tromsø based research
company, Akvaplan-niva AS. Central in Imsland‘s research is
the internal and external factors that control the growth and
maturation process in fish, including how these are controlled
by the genetic background of the fish and/or controlled by
environmental factors. He has published more than 170 peer
review papers in reputed journals.
Genomics of lumpfish (Cyclopterus lumpus): towards marker
assisted selection
Albert K D Imsland2,3, Simo N Maduna1 , Adam Vivian Smith1,
Olof Dora Bartels Jonsdottir2 and Snorre B Hagen1
1Norwegian Institute of Bioeconomy Research (NIBIO), Norway
2Akvaplan-niva, Iceland Office, Iceland
3University of Bergen, Norway