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International Journal of Applied Science - Research and Review
ISSN 2394-9988
E u r o p e a n C o n f e r e n c e o n
Agriculture, Horticulture
& Epigenetics
F e b r u a r y 2 5 - 2 6 , 2 0 1 9
P a r i s , F r a n c e
Agriculture & Epigenetics 2019
Balanced nutrition plays an important role in the maintenance of healthy life.
Imbalance in any of it, results in various metabolic disorders. L-Methionine
(L-Met) is one of the essential amino acids which play an important role
in variety of cellular processes. Reports suggest that dietary methionine
restriction as well as its supplementation both have beneficial effects in
animal models. But in long run methionine restriction has prominent adverse
effects on bone, immune system and can cause cardiac adverse event (via
hyperhomocysteinemia). Here, we report the protective effect of L-Met (0.45%
L-Met supplementation in diet) on T2DM induced hyperglycemia, dyslipidemia
and other complications. Interestingly, L-Met supplementation also activates
hepatic p-AMPK and its downstream signaling molecule SIRT1, mimicking
anti-diabetic drug metformin, an AMPK activator. Real Time PCR, results
show that L-Met supplementation prevents diabetes induced increase in
expression of master regulator FOXO1, hepatic DNMT1 expression and global
histone H3K36 di-methylation. Furthermore, FOXO1 regulated genes, involved
in hepatic glucose metabolism and lipogenesis are also modulated by L-Met
supplementation. Chromatin-immunoprecipitation assay shows that L-Met
supplementation decreases the H3k36me2 abundance on FOXO1 promoter.
We provide first evidence for the involvement of epigenetic alterations in
preventing progression of diabetes by L-Met supplementation.
Biography
Umashanker Navik has his completed M Pharmacy
(Pharmacology and Toxicology) from NIPER SAS Nagar, B
Pharmacy fromUniversity Institute of Pharmaceutical Sciences
(UIPS), Panjab University, Chandigarh, India and is currently
associated with Laboratory of Epigenetics and Diseases
at NIPER SAS Nagar as a PhD Scholar. He is working on
L-Methonine induced epigenetic changes in type 2 diabetes
and non-alcoholic steatohepatitis under the supervision of
Prof Kulbhushan Tikoo. He has been trained extensively in
in-vitro, preclinical experimental techniques like handling
laboratory animals, animal surgeries, physiological and
biochemical measurements, histological, molecular biology
(western blotting, RT-qPCR, Bisulphite sequencing, Chromatin
Immunoprecipiation etc.), statistical analysis of experimental
data and scientific data retrieval. Besides, he played a significant
role in carrying out consultancy projects related to T2DM,
NASH and Toxicity studies in GLP-certified National Toxicology
Centre at NIPER SAS Nagar. Further, he is also mentoring junior
researchers in different field CVDs, diabetes, NAFLD, and role
of probiotics in metabolic diseases. His key interest research
areas are evaluation of Pharmacological Interventions,
Targeting Pathophysiological Cascades in metabolic diseases
such as obesity, diabetes, non-alcoholic fatty liver disease
and elucidation of its link with epigenetics. He has one paper
published in Indian J Pharmacol.
Assessing the effects of dietary L-Methionine supplementation
induced epigenetic alterations in type 2 diabetic rats
Umashanker Navik, Vaibhav G Sheth and Kulbhushan Tikoo
National Institute of Pharmaceutical Education and Research Mohali, India
Umashanker Navik et al., Int J Appl Sci Res Rev 2019, Volume: 6
DOI: 10.21767/2394-9988-C1-008