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International Journal of Applied Science - Research and Review
ISSN 2394-9988
E u r o p e a n C o n f e r e n c e o n
Agriculture, Horticulture
& Epigenetics
F e b r u a r y 2 5 - 2 6 , 2 0 1 9
P a r i s , F r a n c e
Agriculture & Epigenetics 2019
M
ango is a worldwide popular fruit for its excellent delicacy and high
nutritive value. At postharvest stage, the fruit suffers considerable loss
due to anthracnose disease caused by Colletotrichum gloeosporioides. In
this study, we propose a nanotechnology based solution for controlling the
fungal disease without compromising the fruit quality. Sulphur is the choice
of nano-element for its potential fungitoxicity and non-toxicity for human
consumption at low doses. Chemically synthesized sulphur nanoparticles
(SNP) were characterized by XRD analysis and electron microscopy.
In vitro
,
the synthesized SNP showed >50% fungal growth retardation and ~70% fungal
spore germination inhibition at 1 µM concentration. The nanoparticle induced
morphological deformities of fungal mycelia were recorded under scanning
electron microscope (SEM). In vivo, SNP (1µM) was employed to control the
fungal infection on the fruit body. A popular mango cultivar in India namely
Golap Khas was used for this purpose. SNP treated fruits were stored for 7 days
at room temperature and compared to untreated and spore inoculated fruits.
Disease severity of each group of fruits was calculated at regular intervals
during the storage period. At 7th day of storage, SNP treated fruits developed no
symptom of fungal infection while untreated and spore inoculated fruits were
severely affected. Pathogenesis related enzymes like peroxidase, polyphenol
oxidase, phenylalanine ammonia lyase and β-1, 3-glucanase activity and
total polyphenol and flavonoid contents in all stored fruits were measured
for better understanding the SNP induced infection control. However, in all
cases, biochemical activities of SNP treated fruits were much higher than the
untreated fruits which may be attributed as SNP induced biochemical boost of
the fruit body resulting better immunity against fungal pathogens. This is the
very first initiative to exploit SNP in postharvest management which can be
extended to control fungal infection in many more fruits and vegetables.
Biography
Khushi Mukherjee has earned her PhD from University of
Calcutta, India in the year 2012 and has completed his
Postdoctoral research from National Institute of Technology,
Rourkela, India in 2015. Presently, she is serving as UGC
sponsored Postdoctoral Fellow in University of Calcutta,
India. Her research area comprises nanotechnology based
fungicide formulation facilitating better control over the pre-
and postharvest disease causing fungal pathogens. She has
published a number of research papers in reputed journals and
presented her work inmany national and international seminars
and conferences.
khushi2412@gmail.comSulphur nanoparticles: a promising solution to postharvest
anthracnose disease of mango
Khushi Mukherjee and Krishnendu Acharya
University of Calcutta, India
Khushi Mukherjee et al., Int J Appl Sci Res Rev 2019, Volume: 6
DOI: 10.21767/2394-9988-C1-008