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Advanced Dental Care 2018 Dentistry and Craniofacial Research
ISSN: 2576-392X Page 40
October 08-09, 2018
Moscow, Russia
26th International Conference on
Advanced Dental Care
Background: Predictable regeneration of alveolar bone
defects has always been an important therapeutic challenge
in implant dentistry. Allografts including FDBA and DFDBA are
some substitutes being widely used and reported as having
osteoinductive activities with some degrees of controversy.
Aim: The aim of this study is to determine the effect of growth
factors (GFs) on osteoinductive activities of different bone
materials.
Materials & Methods: MG-63 cells were exposed to 60 mg
amounts of four different commercially available freeze-dried
bone allografts with or without 5 ng/mL of two growth factors
(singular or in combination). After 24 and 72 hours of incubation,
the effect of water-soluble allograft released materials and
soluble growth factors on cell viability and proliferation was
assessed using methyl thiazol tetrazolium (MTT) assay. Cell
differentiation and mineralization was respectively assessed by
real-time quantitative reverse transcription PCR (qRT-PCR) and
alizarin red staining after 72 hours of exposure.
Results: The effect of different GFs on cell/allograft containing
plates was affected by the allograft type. Early proliferative and
late osteoinductive effects of GFs were more consistent in TGF-β
rather than PDGF. PDGF only showed limited osteoinductivity in
terms of accelerating BSP and OC genes.
Conclusions: Based on the results of this study, TGF-β can have
additional osteoinductive effect on allografts/cells combination
and its application may be beneficial in in vitro and clinical
regenerative studies.
Effects of different bone allografts with and
without growth factors on proliferation,
osteogenic differentiation and mineralization of
MG-63 osteoblast-like cells
Surena Vahabi, M Torshaby and A Esmailnejad
Shahid Beheshti Medical University, Iran
Dent Craniofac Res 2018, Volume 3
DOI: 10.21767/2576-392X-C4-012