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Advanced Dental Care 2018 Dentistry and Craniofacial Research
ISSN: 2576-392X Page 37
October 08-09, 2018
Moscow, Russia
26th International Conference on
Advanced Dental Care
Background: Migration through micro-perforated membranes
might help in managing the periodontal defect isolation from
surrounding regenerative elements problem caused by guided
tissue regeneration occlusive membrane. Macro-perforated
membrane affect the membrane mechanical properties and
abolish its barrier effect against gingival epithelium and
extracellular matrix components.
Materials & Methods: Gingival mesenchymal stem cells
were seeded on the upper chambers of collagen-coated
polytetrafluoroethylene trans-wells with readymade pore
diameters of 0.4, 3 and poly carbonic acid trans-wells with
readymade pore diameters of 8 microns. Bovine serum was
added to culturing media in the lower chambers versus plain
media in control groups.Migrated cellswere counted, proliferation
evaluated using MTT assay. Scanning electron microscopic
images of the lower surface of perforated trans-well membranes
were obtained.
Results: Gingival mesenchymal stem cells migrated more
significantly in FBS chemotaxis groups compared to the control
groups. 8 microns perforated membrane groups showed
statistically significant more cell migration compared to 3 and 0.4
microns groups, scanning electronmicroscope images confirmed
cells migration through the perforations.
Conclusions: This study results demonstrated that micro
membrane perforations of 0.4, 3 and 8 microns are suitable pore
diameters for gingival mesenchymal stem cells migration to
chemotacticmedia,andareocclusiveforcellmigrationinnegative
control, without affecting membrane mechanical or occlusive
properties, which can be used to develop GTR membrane with
selective cell migration ability.
In vitro migration dynamics of gingival
mesenchymal stem cells through micro
perforated membranes
Al Bahrawy M1, Gaffar K1, Gamal A1 and Iacono V2
1Ain Shams University, Egypt
2Stony Brook University, USA
Dent Craniofac Res 2018, Volume 3
DOI: 10.21767/2576-392X-C4-012