Dr.Yazan Hussien Akkam
Department of Molecular Biology and Program in Cells, University of Arkansas,USA
2016-2017 : Fellowship in Responsible Conduct of Research, University of California, San Diego-USA .
2008-2013: Ph.D., Program in Cell and Molecular Biology, University of Arkansas, Fayetteville-USA, Dissertation: Design, Development, and Characterization of Novel Antimicrobial Peptides for Pharmaceutical Applications . Advisor: Dr. David McNabb.
2006: B.Sc. of Pharmacy was certified by Educational Credential Evaluators (Equivalence to Bachelor of pharmacy in United State of America)-8/28/2006.
1996-2001: B.Sc., Pharmacy, Jordan University of Science and Technology, Irbid-Jordan.
?Proteomics (Protein Digestion, Identification by 2D/MS,western blot and immunoprecipitation).
?High performance liquid chromatography (HPLC) and Mass Spectrum.
?Trainer and Product Specialist for Bio-Rad instruments:
o Low pressure chromatography LP-with Data View software
o Gel documentation and Densitometer
o Quantity One and PDQuest software
o Capillary Electrophoresis
o Two Dimension-Electrophoresis
o Experion Automated station.
?Microbiology skills: Minimum inhibitory concentration, disc diffusion method and Broth diffusion method.
?Fluorescence and confocal Microscopy
?Enzymology experience; Purification, characterization and kinetics (Km ,Vmax ,Km/Vmax, substrate, etc).
?Molecular Biology:Standard cloning, plasmid construction, PCR, and other techniques with in E.coli, Yeast and Drosophila.
?Yeast 2 hybrids system (split-ubiquitin)
?Physical Biochemistry skills: IR, CD and Multidimensional NMR spectroscopy (TOCSY, NOESY, ROESY, HSQC, HMBC), 3D structure calculation and Isothermal titration calorimetry.
?Peptides and Peptoids: synthesis, purification, modification, characterization and labeling.
?Molecular Mechanics Modeling: Quantum mechanics, Molecular dynamics and Monte Carlo.
?Nanomaterials handling and characterizations; TEM, SEM, DLS, Zeta potenitial , FTIR.