wound-congress-dermatology-congress-2018-posters

2 / 20

Notes:
Volume 4 Clinical Pediatric Dermatology
ISSN: 2472-0143
Page 54
JOINT EVENT
Wound Congress 2018 &
Clinical Dermatology Congress 2018
October 15-16, 2018
October 15-16, 2018 Rome, Italy
&
5th International Conference on
Advances in Skin, Wound Care and Tissue Science
14th International Conference on Clinical Dermatology
Development of in vitro skin models to investigate the effect of biocidal agents on skin infections
Beleid G1, Shepherd J2, Miller K1 and Le Maitre CL1
1Sheffield Hallam University, UK
2University Sheffield, UK
The management of wound infections presents a challenge to healthcare authorities both in terms of economic burden
(Chan, B., et al 2017, Nussbaum SRM, et al 2017) and the need to reduce the use of antibiotics due to the rising global crisis
of antibiotic resistance (Ventola, 2015). This has led to the search for alternative approaches. Previous studies have shown that
ultrasound used in conjunction with antibiotic therapy is more effective than antibiotic treatment alone in reducing bacterial
load (Guirro, 2016, Yu, et al 2012). This study developed a 3D skin model utilizing HaCaT cells and fibroblasts which were
seeded onto de epimerized dermis (DED) This model was then subjected to a controlled burn and infected with S. aureus.
Effects of free radical generating antimicrobial strategies (Maillard, 2002): Low-frequency ultrasound (LFU); Silver nitrate;
2-methyl-4- isothiazoline-3-one and Medical Grade Manuka honey were investigated. The cellular phenotype and toxicity of
biocidal agents on mammalian and bacterial cells were investigated. 3D skin was generated which demonstrated keratinocyte
(Cytokeratin 10 &14) and fibroblast (S100A4) markers and underlying collagen type IV in the DED skin layer. In Bacterial
biofilms, there was marked inhibition of S. aureus (SH1000), P. aeruginosa (NCIMB 8295), S. epidermidis and MRSA growth
with all biocides. A decrease in S. aureus (SH1000) bacteria viability and number of CFU were observed in infected tissue-
engineered skin models. This study describes the development of a well characterized skin model which can be utilized to
develop and test biocidal agents for skin infections.
Biography
Guma Beleid graduated from the Faculty of medical technology, Derna, Libya, with Bsc in Laboratory medicine and MSc in Clinical Microbiology from Academy of
Graduate Studies, Tripoli, Libya. He worked as a lecturer assist in the Faculty of medical technology, Derna, Libya for four years. He is currently a PhD Student in
the Biomolecular Sciences Research Centre at Sheffield Hallam University, Sheffield, UK.
Beleid G et al., Clin Pediatr Dermatol 2018, Volume 4
DOI: 10.21767/2472-0143-C2-006