13 / 20
Page 52
Journal of Clinical Nutrition & Dietetics
ISSN:2472-1921
3
r d
W o r l d C o n g r e s s o n
Nutrition, Dietetics
and Nutraceuticals
F e b r u a r y 2 5 - 2 6 , 2 0 1 9
P r a g u e , C z e c h R e p u b l i c
World Nutrition 2019
Background:
It has been reported that vitamin D deficiency is more prevalent in people with diabetes than healthy population
and might be responsible for worsening of diabetes complications. This study was designed to investigate the effect of vitamin
D treatment on the expression of five key genes involved in the development of vascular complications in heart, liver and kidney
tissues.
Methods:
Twenty-four male Sprague–Dawley rats were randomly divided into three groups. The first group served as control
and the other two groups received an intraperitoneal injection of 45 mg/kg STZ to develop diabetes. Then groups were treated
for four weeks either with placebo or vitamin D (two injections of 20,000 IU/kg). At the end of the experiment, blood levels of
glucose, insulin, HbA1c and advanced glycation end products (AGEs) were measured. Tissue samples were assessed for the
gene expression of AGE cellular receptor (RAGE), glyoxalase (GLO), aldose reductase (AR), O-GlcNAc transferase (OGT) and
glutamine: fructose-6-phosphate aminotransferase (GFAT).
Results:
Vitamin D treatment resulted in a significant increase in insulin concentration, which could improve hyperglycaemia in
diabetic rats. HbA1c concentration had a slight but insignificant decrease following vitamin D intake. In addition, a significant
reduction was observed in gene expression of RAGE (the main receptor of AGE pathway) and OGT (the crucial enzyme in the
hexosamine pathway) in heart and kidney, as well as GFAT (the rate limiting enzyme of the hexosamine pathway) in all tissues.
Conclusion:
Vitamin D might contribute in reducing diabetes complications not only by improving blood glucose and insulin
levels, but also via modulating AGE and hexosamine pathways in different organs.
h.derakhshanian@gmail.comThe preventive role of vitamin D in diabetes
complications: exploring cellular pathways
Hoda Derakhshanian
1
, A Djazayery
2
, M H Javanbakht
2
,
M R Eshraghian
2
, A Mirshafiey
2
and M Djalali
2
1
Dietary Supplements and Probiotic Research Center-Alborz University of Medical Sciences, Iran
2
Tehran University of Medical Sciences, Iran
J Clin Nutr Diet 2019, Volume: 5
DOI: 10.4172/2472-1921-C1-006