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Journal of Clinical Immunology and Allergy
ISSN: 2471-304X
E u r o p e a n C o n g r e s s o n
Vaccines & Vaccination
and Gynecologic Oncology
Vaccines & Vaccination and Gynecologic Oncology 2018
O c t o b e r 2 6 - 2 7 , 2 0 1 8
B u d a p e s t , H u n g a r y
A
ge-related decline in immune responses of geriatric horses is associated with a reduced response to vaccination and
increased susceptibility to equine influenza virus (EIV) infection. The aim of the present study was to compare and evaluate
the immune responses of geriatric horses given 2 doses of ‘live’ or ‘killed’ EIV vaccines. 27 geriatric horses (>20 yrs) were used in
this study, all of which had prior exposure to equine influenza (EI) vaccination or infection. Treatment groups were stratified based
on HI titres and body condition scores: Group 1 (n=7) ‘killed’ Calvenza™ EIV vaccine (OH/03 and KY/95, and Newmarket/93),
Group 2 (n=7) inactivated Fluvac Innovator® EIV vaccine (KY/97) and Group 3 (n=7) ‘live’ canarypox vectored Recombitek® EIV
vaccine (KY/94 and Newmarket/93). Group 4 (n=6) served as the non-vaccinate controls. Serum samples were collected prior to
vaccination (day 0) and on days 7 and 14 post vaccination. 14 days (day 28) after the first vaccination, a second vaccination was
administered, and serum samples were collected on days 35 and 42. Antibody responses were measured by HI, SRH and ELISA.
Results showed that no significant difference (P>0.05) in HI, SRH and ELISA antibody titres for the control horses throughout the
study. Post vaccination, there was a significant (P<0.05) increase in HI, SRH and ELISA antibody titres for all three vaccine groups
between day 0 and day 7. No significant difference (P<0.05) was seen for groups between day 7 and 14, or between day 14 and
21, or between day 21 and 28 (2
nd
vaccination). Both ‘killed’ vaccines induced a significantly (P<0.05) higher antibody response
measured via HI, SRH and ELISA when compared to the ‘live’ canarypox vectored vaccine. Our results show that whilst aged
horses possess baseline antibody titres to EIV antigen through previous infection and or vaccination, vaccination with either killed
or live vaccines can boost this response.
melslean@hotmail.comHumoral immune responses to commercially
available equine influenza vaccines in aged horses
Lean M S
1
, Reedy S E
2
, Chambers T M
2
, Betancourt A
2
, Horohov
D W
2
and Adams A A
2
1
Aberystwyth University-IBERS, UK
2
GLUCK Equine Research Centre, University of Kentucky, USA
Journal of Clinical Immunology and Allergy, Volume: 4
DOI: 10.21767/2471-304X-C2-006
Euro Vaccines 2018