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E u r o S c i C o n c o n f e r e n c e o n
Protein, Proteomics and
Computational Biology
Biochemistry & Molecular Biology Journal
ISSN: 2471-8084
D e c e m b e r 0 6 - 0 7 , 2 0 1 8
Am s t e r d a m , N e t h e r l a n d s
Proteomics and Computational Biology 2018
Page 15
A
rapid,sensitiveandaccurateresponseofanalyticaltechniquestoresolvehealth
issues, such as low limit identification in special biological environment (i.e.
complexmixturesof proteins) remains tobeingakeyaspect inmodernproteomics.
In fact, extensive efforts to obtain sensitivity enhancements with detection limits
even down to the single molecule have been devoted in the nanotechnology
framework. Recently, innovative approaches based on the properties of colloidal
nanoparticle (NP) assemblies have led to the development of novel diagnostic
methods with sensitivity enhancements for single molecule protein monitoring/
identification/detection. Among them, surface-enhanced Raman spectroscopy
(SERS) benefits from its higher detectable response to binding of a single protein
(sensitivity) and also, very importantly, from its multiplexing capabilities due
to the narrow nature of detected peaks from Raman reporter molecules. Since
SERS retains the fingerprinting capabilities of Raman spectra, the internal modes
of a reporter molecule brought at metallic NPs junctions, where strong field
enhancement occurs, can be used as diagnostic tools. Specific attention has
been given to SERS-based immunoassays. Indeed, the combination of the high
sensitivity provided by SERS and the strong binding specificity of antibody-protein
ensures that SERS-based detection platform are suitable tools for biomedical and
biochemical analysis, clinical diagnosis and biosensor. Therefore, the superior
capabilities of SERS readout strategy such as high sensitivity and simultaneous
detection of a multiple proteins in complex matrices will be highlighted in this
presentation.
Biography
Nekane Guarrotxena has completed her PhD in Chemistry
from Complutense University, Madrid-Spain and Postdoctoral
research positions from ENSAM, Paris-France and University
of Science II, Montpellier-France. She was the Vice-Director of
the Institute of Polymer Science and Technology (ICTP-CSIC)
(2001-2005), a Visiting Professor in CPOS of University of Cali-
fornia, Santa Barbara-US and CaSTL of University of California,
Irvine-US (2008-2011). She has published in more than 60 pa-
pers in reputed journals, four books (also co-editor) and 24 book
chapters, and has been serving as an Editorial Board Member
of repute, an Organizing Committee Member of scientific and
technological events, and an External Expertise Consultant on
I+D+I Management and Policy for National and International
Agencies. Her research interest focuses on the synthesis and
control-assembling of hybrid nanomaterials, smart-stimuli
nanostructures, nanoplasmonics and their applications in bio-
imaging, drug delivery, therapy and sensing.
nekane@ictp.csic.esSensitive and multiplexed response of SERS-
based plasmonic nano-dumbbell platforms in
disease biomarkers diagnostics
Nekane Guarrotxena
Institute of Polymer Science and Technology-Spanish National Research
Council (ICTP-CSIC), Spain
Nekane Guarrotxena, Biochem Mol biol J Volume:4
DOI: 10.21767/2471-8084-C5-019