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Volume 4 Nano Research & Applications
ISSN: 2471-9838
Page 77
JOINT EVENT
August 16-18, 2018 | Dublin, Ireland
&
12th Edition of International Conference on
Nanopharmaceutics and Advanced Drug Delivery
25th Nano Congress for Future Advancements
Nano Congress 2018
&
Nano Drug Delivery 2018
August 16-18, 2018
Ananostrategy for characterizing the phenotypic evolution of circulating tumor cells during therapy
Jing Wang1, Yuling Wang2 and Matt Trau1,3
1Australian Institute for Bioengineering and Nanotechnology - The University of Queensland, Australia
2Macquarie University, Australia
3The University of Queensland, Australia
Targeted therapies have been proved to be effective in cancer treatment but are limited by the rapid acquisition of drug
resistance (within months). A rapid and non-invasive method to monitor drug response would promote precision
medicine and improve treatment efficacy. Circulating tumour cell (CTC) analysis has emerged as a useful monitoring tool, but
its routine usage is restricted by either limited multiplexing capability or sensitivity. Here we demonstrate the use of antibody-
conjugated and Raman reporter-coated gold nanoparticles for simultaneous labelling and monitoring of multiple CTC surface
markers (named as “cell signature”), without the need for isolating individual CTCs. Our nanostrategy is capable of detecting
10 tumor cells in 10 mL of blood. We also observe cell heterogeneity and phenotypic changes of melanoma tumor cells during
molecular targeted treatment. Furthermore, we follow the CTC phenotypic changes of 10 stage-IVmelanoma patients receiving
immunological or molecular targeted therapies. Our technique maps the phenotypic evolution of patient CTCs and shows
drug-resistant clones having different CTC signatures of potential clinical value. We believe our proposed method is of general
interest in the CTC relevant research and translation fields.
Nano Res Appl 2018, Volume 4
DOI: 10.21767/2471-9838-C3-015