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Volume 3, Issue 2 (Suppl)
Med Mycol Open Access
ISSN: 2471-8521
Mycology 2017
September 25-26, 2017
Page 38
Notes:
conference
series
.com
September 25-26, 2017 Chicago, USA
2
nd
International Conference on
Mycology & Mushrooms
Comparative study on whole genome sequences of
Aspergillus terreus
(soil fungus) and
Diaporthe ampelina
(endophytic fungus) with reference to lovastatin production
L
ovastatin is a competitive inhibitor of the enzyme hydroxymethyl glutaryl coenzyme A reductase (HMGR) in cholesterol
biosynthetic pathway and hence used in the treatment of hyperlipidemia. In a previous study, we report a tropical soil
isolate,
Aspergillus terreus
(KM017963), which produces ample amount of lovastatin than its counterpart, which are endophytic
in origin. Bioinformatic analysis of whole genome sequence of
A. terreus
(AH007774.1), a soil isolate revealed the presence
of gene cluster responsible for lovastatin production, whereas, endophytic fungi including a species of
A. terreus
showed no
homology with the lovastatin gene cluster. The molecular study was also carried out targeting PCR amplification of the two
important genes,
lov
E (a regulatory gene) and
lov
F (transcriptional regulatory factor) in genomic and c-DNA of soil and
endophytic fungi. Expression of the two genes was successful in
A. terreus
(KM017963), whereas the same was not achieved
in endophytic fungi. To further validate our above findings, in the present study, the whole genome sequencing of
A. terreus
and a selected endophytic fungus,
Diaporthe ampelina
(Phomopsis) was performed. Lovastatin gene cluster, when aligned on
the consensus sequence of both genomes, the entire lovastatin gene cluster was detected in a single scaffold (1.16) of
A. terreus
genome. On the contrary, there was a complete absence of lovastatin gene cluster in the genome of
D. ampelina
(an endophyte).
The probable reasons for the absence of lovastatin gene cluster in endophytic fungi will be discussed.
Biography
Savitha Janakiraman has received her PhD degree in Botany, Madras University and postdoctoral research in University of Hull, England under the Jawaharlal
Nehru (UK) fellowship. Currently, she is working as Professor in Bangalore University, Department of Microbiology. She is the Chairman of Board of Examination
(BOE) and Member of Board of syllabus and Academic Council of Bangalore University. She has published several research papers and has several research
projects offered by Government funding agencies. Her interest of research is on Industrial Microbiology and Environmental Microbiology.
drsvtj@yahoo.co.inSavitha Janakiraman
Bangalore University, India
Savitha Janakiraman, Med Mycol Open Access, 3:2, 2017
DOI: 10.21767/2471-8521-C1-001