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Immunology 2018
J u l y 0 5 - 0 7 , 2 0 1 8
V i e n n a , A u s t r i a
Page 77
Journal of Clinical Immunology and Allergy
ISSN 2471-304X
1 5
t h
I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology
The transition elements: chromium and nickel are essential
micronutrients for human, animals and plants. Micronutrients in human
and animal organisms play crucial role in prevention and treatment of
various diseases. They also play an important role in the optimization
of physical and mental functions. Inside the cells, chromium (III) may
interact with microfilaments, mitochondria, lysosomes and nucleus.
Cr (III) compounds can bind directly to DNA
in vitro,
forming Cr-DNA
adducts and DNA-DNA crosslinks. Nickel can induce genotoxic effects,
such as DNA strand breaks, sister chromatin exchanges and DNA-
protein crosslinks. Moreover, nickel can generate ROS production,
which interacts with nucleic acids or proteins. The aim of this study was
to examine the effect of treating of chromium (III) and nickel (II) and
their combinations on Bovine Viral Diarrhoea Virus (BVDV) replication.
The BVDV has been a good model virus for investigating HCV, which
is a member of genus
Hepacivirus
, which belongs to the same family.
The antiviral efficacies of chromium (III) and nickel (II) on BVDV were
evaluated using Real Time PCR method. Moreover, the cytotoxicity of
these microelements was examined using the MTT reduction assay.
The IC
50
(50% inhibitory concentration) for the chromium chloride was
1400 µM for BT cells. The IC
50
for the nickel chloride was more than
1200 µM for BT cells. The concentration-dependent antiviral activity
of chromium chloride and nickel chloride against BVDV was observed.
In cultures simultaneously treated with 1) 200 μM of CrCl
3
and 1000
µM of NiCl
2
, 2) 1000 μM of CrCl
3
and 200 µM of NiCl
2
, a decrease in
number of RNA copies was observed compared with control cells
and cells incubated with chromium(III) and iron(III) used separately.
The synergistic antiviral effects were observed for chromium (III) and
nickel (III) against BVDV.
Regulation of chromium (III) and nickel (II) of RNA virus
replication
Sylwia Terpiłowska
1
and Andrzej K Siwicki
2
1
The John Paul II Catholic University of Lublin, Poland
2
University Warmia and Mazury in Olsztyn, Poland
Biography
Professor Andrzej K. Siwicki, VMD, Ph.D., DSc is a head of Department
Microbiology and Clinical Immunology, Faculty of Veterinary Medicine,
University of Warmia and Mazury and Department of Pathology and
Immunology IFI in Olsztyn, Poland. He is author of over 500 original papers
in reputed journals and about 200 scientific communications (index-h 32,
index of citation: 4502). His fields of interest are modulation of defence
mechanisms and protection against diseases by natural and synthetic
products in animals, influence of pollutants on the cell-mediated immunity
and restoration of immunity after suppression induced by xenobiotics.
Developed a new possibility in the comparative immunotoxicology for
control of effect of xenobiotics and pharmaceutical products on the
defence mechanisms and protection against diseases. He attended
scientific missions in USA, Japan, France and Israel, was a co-ordinator
of USDA and FAO projects and he is professor conferring of 14 PhDs and
4 DSc.v.
siwicki@uwm.edu.plAndrzej K. Siwicki et al., Insights Allergy Asthma Bronchitis 2018, Volume: 4
DOI: 10.21767/2471-304X-C1-003