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Volume 2, Issue 1
Neurosurg
ISSN: 2471-9633
Global Stroke 2017
July 05-06, 2017
Neurodegenerative Disorders and Stroke
July 05-06, 2017 Frankfurt, Germany
4
th
International Conference on
Iron homeostasis and hepcidin quantification in Alzheimer’s disease patients
Manolov V
1
, Hadjidekova S
1
, Petrova J
1
, Vasilev V
2
, Petrova M
1
, Kuntchev T
1
, Jelev Y
1
, Jeliazkov P
1
, Tzatchev K
1
and
Traykov L
1
1
Medical University Sofia, Bulgaria
2
Aleksandrovska University Hospital, Bulgaria
Background:
Alzheimer’s disease (AD) is characterized by deposition of amyloid plaques of amyloid-β chelating peptide with
transition metal ions (Cu
2+
, Zn
2+
и Fe
3+
). The binding of Cu
2+
and Fe
3+
leads to toxic chemical reactions; a change in the oxidation of
two metals, that leads to H
2
O
2
production in the presence of transition metals and finally gives toxic free OH• radicals.
Methods:
41 Alzheimer’s disease patients were included in this study. They were evaluated for serum iron, copper, selenium, zinc
and hepcidin levels. Superoxide dismutase (SOD) and glutathione peroxidase (GPX) were measured as oxidative stress markers.
Hepcidin, SOD and GPX were measured by ELISA methods. Serum Fe, Cu, Se and Zn were quantified by AAS. The results form AD
patients were compared to age and gender matched healthy controls. We used Pearson’s correlation and Student’s paired t-test for
statistical analysis of established results.
Results:
We found statistically significant elevated serum iron, copper and zinc results in AD patients (42.9 µmol/l, 39.7 µmol/l, and
41.1 µmol/l) compared to control group (21.5 µmol/l, 18.7 µmol/l, and 15.9 µmol/l); P<0.01. Plasma selenium levels were decreased
in AD patients (121.7 nmol/L) compared to healthy controls (652.4 nmol/L); P<0.005. Hepcidin concentrations were increased in AD
cases (61.1 µg/l) compared to controls (20.7 µg/l); P<0.001. SOD and GPX levels were decreased in Alzheimer’s disease (8.9 µg/ml,
and 11.4 pg/mL) compared to normal values in healthy controls (21.7 µg/ml; and 39.5 pg/mL); P<0.001.
Conclusions:
The expected contribution from our study is practical introduction of quantification of serum hepcidin as a potential
marker for early diagnosis of impaired iron homeostasis, leading trace element in the pathogenesis of neurodegenerative diseases.
Biography
Manolov V has completed his PhD from Medical University in Sofia, Bulgaria. He is working as Assist. Prof. at Department of Clinical laboratory and clinical
immunology at the same University. He has interests in iron metabolism, gynecology, neurology, endocrinology and pediatrics. He has published more than 25
papers in reputed journals and participated in more than 60 National and International meetings in different medicine fields.
victhedoc3@mail.bgManolov V et al., Neurosurg 2017, 2:1
DOI: 10.21767/2471-9633-C1-002