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Volume 9
Journal of Neurology and Neuroscience
ISSN: 2171-6625
Page 71
JOINT EVENT
July 23-24, 2018 Birmingham, UK
&
24
th
International Conference on
Neuroscience and Neurochemistry
26
th
Edition of International Conference on
Clinical Psychology and Neuroscience
Short-term local hypothermia prevents ischemia-reperfusion injury following delayed tissue
plasminogen activator treatment in an embolic stroke model
Mohammadreza Zarisfi
Rafsanjan University of Medical Sciences, Iran
Background:
Limiting reperfusion injury immediately after delayed tissue plasminogen activator (tPA) therapy in cases of
acute ischemic stroke seems to be beneficial for extending the time window of this drug. The present study sought to determine
whether short-term, mild local brain cooling can prevent hyperemia and/or adverse effects of delayed tPA in a rat embolic
stroke model.
Materials &Methods:
Male wistar rats were subjected to embolic stroke using homologous clots and randomly assigned to one
of the following conditions: control, tPA (10 mg/kg; i.v.), local hypothermia (LH), and tPA + LH (10 mg/kg; i.v.). The tPA was
injected at 6 h following embolic stroke. LH was conducted at 6.5 h after ischemia and maintained thereafter for approximately
30 min. Cerebral blood flow was evaluated for 60 min, starting from the time of tPA injection. Infarct volume, blood–brain
barrier (BBB) disruption, brain edema, neurological deficits, and the serum level of matrix metalloproteinase-9 (MMP-9) were
measured 48 h later.
Results:
Compared to the tPA and control groups, the combination of tPA + LH significantly reduced infarct volume (P<0.001
and P<0.05, respectively). tPA significantly increased rCBF at approximately 30mins after administration (P<0.001) but
applying LH at 30 min after tPA injection not only prevented the increase of rCBF but caused a 20% decrease in reperfusion
compared to the control and tPA groups (P<0.001). The combination of LH + tPA reduced BBB leakage (P<0.001), MMP-9
level, and brain edema (P<0.01). LH alone also decreased BBB disruption (P<0.01) and brain edema (P<0.05). Moreover, the
combination of LH + tPA decreased neurological deficits at 48 h following stroke (P<0.01) and increased grasping ability and
sensory-motor function (P<0.001).
Conclusion:
The application of short-term local hypothermia is a promising strategy to mitigate reperfusion injuries following
delayed tPA therapy and to extend its time window up to 6 hrs.
zarisfi_mr@yahoo.comJ Neurol Neurosci 2018, Volume 9
DOI: 10.21767/2171-6625-C2-012