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Pan Zhao

Pan Zhao Pan Zhao

Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH, USA.

 
Biography

postdoctoral scholar, working in Dr. Walter Boron’s Lab in Department of Physiology and Biophysics at Case Western Reserve University. Completed PhD in 2010 from department of physiology, Peking University, Beijing, P. R. China. In 2005, she received Award for Excellence in Teaching, from department of pathophysiology, Zhengzhou University, China. She is a member of American Physiological Society (APS, 2015-present).

 
Research Interest

Research work is now focused predominantly on (1) identifying and characterizing the membrane proteins that serve as oxygen channels in red blood cells (RBCs) by using stopped-flow (SF) absorbance spectroscopy to determine the O2 saturation of hemoglobin (Hb). So far, I have completed development a novel stopped-flow (SF) assay for quantitating carbonic-anhydrase activity and assessing red-blood-cell hemolysis, using OOE CO2/ HCO3 solutions. My research area also included (2) exploring the pathogenic and therapeutic roles of metabolic nuclear receptors (PPARs) in renal disease. There are two parts: (i) evaluates the renoprotective role of peroxisome proliferator-activated receptor-alpha (PPARα) in doxorubicin-induced glomerular injury, (ii) evaluates the role of nuclear receptor peroxisome proliferators-activated receptor β/δ (PPARβ/δ) in IL-1β-induced renal inflammation in human mesangial cells (hMCs). (3) exploring mechanisms of anti-diabetic agonsts targeting at peroxisome proliferator-activated receptors (PPARs). My experimental techniques include: stopped-flow technique; Cell and Molecular biology (including Agarose gel electrophoresis, PCR, DNA/RNA extract, Isolation of cell nuclear and cytosol, RT-PCR, RT-PCR, Cloning, Western blot, EMSA, Immunoprecipitation; Cell culture of cell-lines and primary cell cultures, Cell transient transfection and luciferase assay, Adenoviruses amplification and infection, ELISA, Flow Cytometry for cell cycles and apoptosis assay; Morphology technology: Immunohistochemistry, Immunocytochemistry, digital confocal imaging, Immunofluorescence, Hematoxylin-eosin (HE) staining, Oil red staining and Experimental animal models and gene knockout mouse management: Mouse gavage, Experimental mouse inflammatory bowel disease (IBD) model, Genotyping.

 

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