Dr. Ao LiVisiting Associate Professor,Department of Pharmacology
,Yale University School of Medicine,USA.
Biography
EDUCATION:
2008.9-2013.7: Peking Union Medical College, Tsinghua University, Beijing, P.R. China. Ph.D., Genetics, Advisor: Dr. Guanqing Wu
2011.6-2012.6: Vanderbilt University, Nashville, TN. Joint Ph.D. Student supported by China Scholarship Council
2004.9-2008.7: Beijing Normal University, Beijing, P.R. China. B.S., Biotechnology.
PROFESSIONAL EXPERIENCE:
2016.12-Present Visiting Associate Professor, Department of Pharmacology, Yale University School of Medicine
2013.8-2016.11 Assistant Professor, Division of Translational Cancer Research and Therapy, Cancer Institute and Cancer Hospital, Chinese Academy of Medical Sciences
Research Interest
1. Molecular Biology:
Nucleic acid extraction from tissue, cell
Genomic PCR, RT-PCR, cloning full length genes by PCR
Gene cloning and expression in E. coli and Yeast system
Southern hybridization, northern hybridization, Colony and In situ hybridization
Design of PCR primers, DNA arrays and analysis of DNA sequence
Site directed mutagenesis
Yeast two-hybrid assay
Generation of knockin, konckout and transgenic construct
2. Bio Chemistry:
Fusion protein design, expression, proteolytic cleavage and purification
Electrophoresis, western blotting, immunoprecipitation and pull down
3. Cell biology:
Cell culture
Primary Cell culture
3D Culture
Transfection
RNAi (with siRNA, shRNA, and lentivirus)
Production of polyclonal antibody in Rabbit
Production of monoclonal antibody in Mouse
4. Immunology:
Immunohistochemistry techniques
Immunofluorescence techniques
Purification of polyclonal antibody
ELISA
5. Animal experiment:
Animal anesthesia
Intravenous