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Biochem Mol biol J
ISSN: 2471-8084
Volume 3, Issue 2
Metabolomics Conference 2017
August 29-30, 2017 Prague, Czech Republic
9th International Conference and Exhibition on
Metabolomics and Systems Biology
Notes:
Page 21
Stable isotope-assisted metabolomics of
Fusarium head blight on wheat
Rainer Schuhmacher, Christoph Bueschl, Asja Ceranic, Maria
Doppler, Bernhard Kluger, Rudolf Krska, Alexandra Simader,
Bernhard Wolf, Barbara Steiner, Hermann Buerstmayr, Marc
Lemmens and Gerhard Adam
University of Natural Resources and Life Sciences, Austria
Metabolomics studies show great potential to provide
an improved understanding of the molecular
mechanisms underlying plant diseases such as Fusarium
head blight (FHB) on wheat. Here, we present the
successful combination of LC-HRMS based global 13C
labeling- & 13C tracer techniques to probe both the attack of
the mycotoxigenic fungus Fusarium graminearum as well
as the metabolic response of near isogenic wheat lines,
differing in a major resistance QTL against FHB. To do so,
parent and corresponding wheat NILs were cultured in the
greenhouse and treated with F. graminearum spores at
anthesis. In addition, custom-tailored, globally 13C-labelled
wheat plants of the resistant and susceptible parent lines
were grown as a reference in a tailor-made labeling
chamber. To further elucidate the phenylalanine (Phe)-
and tryptophan (Trp)-derived submetabolome, wheat
ears were also treated with U-13C phenylalanine or U- 13C
tryptophan (Trp) under control- and infection conditions.
LC-HRMS and subsequent data analysis clearly revealed
some 1.000 metabolites in the tested biological samples.
Among those ca. 100 and 70 were found to be derived
from the metabolic precursors Phe and Trp, respectively.
Substance with levels, which were significantly affected
by Fusarium have been further investigated for their
putative role in QTL mediated resistance against FHB.
The abundance of various substance classes differed
significantly between the tested wheat lines with respect
to both the timing of formation and relative amount. Our
results for hydroxycinnamic acid amides, phenolic acids,
flavonoids and lignans suggest that different molecular
mechanisms contribute to defense and resistance against
FHB. In this talk, I will present the isotope-assisted
metabolomics protocols which we have developed
and how we have investigated defense responses and
resistance mechanisms of wheat against Fusarium head
blight.
Biography
Rainer Schuhmacher is an Associate Professor at University of Natural
Resources and Life Sciences (BOKU), Vienna where he is heading the
working group Metabolomics and Bioactive Compounds. His research focuses
on LC-HRMS and GC-MS based metabolomics of microbes and plants
with a special focus on the interaction between these living organisms. He
received his degrees in Chemistry at University of Konstanz, Germany and
Vienna University of Technology, and in 2009 he completed his Habilitation in
Analytical Chemistry at BOKU University, Vienna. He is co-author of more than
120 SCI publications.
Rainer Schuhmacher et al., Biochem Mol biol J, 3:2
DOI: 10.21767/2471-8084-C1-002