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Volume 3, Issue 4 (Suppl)
Polym Sci
ISSN: 2471-9935 Polym Sci, an open access journal
October 12-13, 2017 Osaka, Japan
Annual Meeting on
Biopolymers and Drug Delivery Systems
Biopolymers Meeting 2017
October 12-13 2017
Page 28
Self-assembled tripeptide fibers as prebiotic RNA binders
Tony Z Jia
Tokyo Institute of Technology, Japan
B
efore the emergence of the first RNA polymerase ribozyme, sustained non-enzymatic replication of RNA was crucial to the
development of early life. To that end, cationic peptides have been hypothesized to be able to assist in template-directed non-
enzymatic RNA polymerization by binding to and selectively inhibiting the annealing of long, complementary RNA strands. These
strands can then act as templates for free primer strands, resulting in progressive replicative turnovers. However, the geochemical
scenarios that would allow for the accumulation of high concentrations of long, cationic peptide oligomers are not likely. Recently,
it has been discovered that certain tripeptide systems can spontaneously self-assemble into hydrogels and fibrillar macrostructures.
As the accumulation of high concentrations of peptide trimers is much more likely than longer oligopeptides on the early earth, we
probed the self-assembly properties and RNA-binding affinities of six tripeptides, each containing a cationic residue in the N-terminal
position followed by an aromatic or hydrophobic dyad. KYF and RFF showed the best fibrillar self-assembly propensity-RFF is the
first purely-peptidic arginine-containing tripeptide to self-assemble and microscopy and binding assays show that RNA indeed binds
to the structures generated by these tripeptides. In fact, binding of a fluorescent RNA to cationic fibrillar macrostructures resulted in
a direct label-free method of visualizing the kinetics of nanostructure assembly in real time. As these tripeptide assemblies are also
reasonably heat-stable and do not prohibitively inhibit nonenzymatic RNA replication, our studies suggest that cationic tripeptide
nanostructures could have been prebiotically-plausible RNA-binders, potentially possessing the ability to assist in the replication and
eventual evolution of early genetic systems.
Biography
Tony Z Jia is a Researcher of the Earth-Life Science Institute at the Tokyo Institute of Technology in Tokyo, Japan. His research interests include prebiotic and
ancient biopolymer formation and evolution.
tzjia@elsi.jpTony Z Jia, Polym Sci 2017, 3:4
DOI: 10.4172/2471-9935-C1-005