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Caffeine prenatal exposure caused persistent alterations in astrocyte morphology in experimental models

Joint Event on 26th Edition of International Conference on Clinical Psychology and Neuroscience & 24th International Conference on Neuroscience and Neurochemistry
July 23-24, 2018 Birmingham, UK

Owolabi Joshua Oladele, Adefule A K and Shallie P D

Babcock University, Nigeria Yale University, USA University of Liberia, Liberia Olabisi Onabanjo University, Nigeria

ScientificTracks Abstracts: J Neurol Neurosci

DOI: 10.21767/2171-6625-C2-011


There are concerns over the consumption of caffeine during pregnancy and early years of life due to the susceptibility of the brain to the stimulant. The aim of this investigation is to assess the effects of pre and postnatal caffeine ingestion on the development of the frontal cortex of mice models. A total of 32 (n=32) adult mice (Mus musculus) were recruited primarily for the study. They were divided into four groups labelled A, B, C and D which include the Control, Low-Dose, Medium- Dose and High-Dose groups respectively. Treatment duration had Phase I and Phase II to assess pre and postnatal effects respectively. In Phase I, Groups B, C and D were given 10mg/kg, 50mg/kg 120 mg/kg body weight of water-dissolved caffeine respectively while Group A animals served as the control. Mating was allowed and the pregnant female mice were administered caffeine from the day 0 (E0) of pregnancy until parturition. Half of the offspring were sacrificed at birth. The remaining offspring from the Phase I experimental animals were recruited into the phase II for postnatal caffeine administration. Phase II [postnatal] treatment started from parturition until postnatal Day 35 marking the average age of puberty. Brain specimens were excised and processed at the end of Phase I and Phase II. Histo and immunochemical properties of the frontal cortex were demonstrated using the Feulgen DNA and GFAP techniques. High caffeine dosage administration during pregnancy produced general cytological and histoarchitectural disruptions and distortions that affected glia as well as other cell types. Cell staining patterns are largely heterogeneous as much as they are in morphological appearances. Astrocytes, staining positive for GFAP, had altered morphologies. Processes are quite sparse and hardly readily observable. These observations indicate possible limitations or retardations in astrocyte differentiation as a result of caffeine effects. Recent Publications 1. Owolabi J O, Olatunji S Y and Olanrewaju A J (2017) Caffeine and Cannabis Effects on Vital Neurotransmitters and Enzymes in the Brain Tissue of Juvenile Experimental Rats. Ann Neurosci. 24:65–73. 2. Owolabi Joshua O, Olatunji Sunday Y, Olanrawaju John A and Ajibade Testimony P (2017) 3. Caffeine exposure at puberty: effects on hippocampal structure, neurochemistry and short term memory in experimental wistar rats. Annals of Experimental Biology 5(3):37-43. 4. Owolabi Joshua Oladele, Olatunji Sunday Yinka, Olanrawaju John Afees and Jimoh-Enesi Queen Amina (2017) Brain-choroid plexus chemistry changes linkable to caffeine ingestion and accompanying short-term memory disturbances in experimental models. American Journal of Laboratory Medicine 2(4):45-58. 5. Owolabi Joshua Oladele, Olatunji Sunday Yinka, Olanrawaju John Afees and Jimoh-Enesi Queen Amina (2017) Histomorphological observations of caffeine and honey effects on choroid plexus. iosr jnhs. journal of nursing and health science (IOSR-JNHS). 6(5):58-66. 6. Olatunji Sunday Yinka, Owolabi Joshua Oladele and Olanrewaju Afees John (2017) Excessive oral intake caffeine altered cerebral cortex histoarchitecture and cell morphologies in adult mice. Anatomy Journal of Africa. 6(1):856- 861.


Owolabi Joshua Oladele has completed his PhD in the field of Anatomy and Neuroscience, with special research focus of toxicology and teratology associated with psychoactive substances. He also has an MBA with specialisation in Clinical Research Management. He has more than 80 publications including articles in reputed journals and books..

E-mail: [email protected]

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