Figure 5.Short circuit current trace from a Calu-3 monolayer showing the responses to forskolin, 1-EBIO and CTX along with the corresponding Nyquist plots under each of these conditions. Plotted are the real (ZR) and imaginary (Zi) components of the impedance at 100 sine wave frequencies over a range of 1 Hz to 22 kHz. The solid line is the fit obtained using the equations that describe the equivalent electric circuit shown in Figure 4. Values for RS, RP, Cap and Cbl were 24 W cm2, 310 W cm2, 6 μF/cm2 and 18 μF/cm2, respectively.