Figure 5.RT-PCR analysis of the mRNA components of the renin-angiotensin system in the pancreatic acinar AR42J cell line. DNase-treated total RNA from AR42J cells was transcribed with (+) or without (-) reverse transcriptase (RT) and amplified with primers specific for rat renin, angiotensinogen (Aogen), angiotensin converting enzyme (ACE), and the angiotensin II receptor subtypes AT1a, AT1b, and AT2.