Figure 4. Effect of dexamethasone (DEX) and 17betaestradiol (estrogen) on AT2 mRNA concentration in the pancreatic acinar AR42J cell line. Top panel: Autoradiogram of the amplified products using primers specific for the rat AT2 receptor and the elongation factor-1alpha (EF1a). Data are from 3 sets of cells untreated (Control, lanes 1-3), treated for 24 hrs with 1 μM estrogen (lanes 4-6) or 1 μM dexamethasone (lanes 7-9). Bottom panel: Densitometric quantification of the AT2/EF1a mRNA; mean+SEM.