Figure 3.Effect of steroid treatment on AT2 binding in the pancreatic acinar AR42J cell line. Cells were treated with 1 μM of dexamethasone (DEX), cortisol, aldosterone or 17beta-estradiol (estrogen) for up to 48 hours. AT2 binding was determined with 125I- [Sar1,Thre8]-angiotensin II in the presence of the AT1 antagonist losartan in intact cells. Data are the mean+SEM from 3 separate experiments. Adapted from Chappell MC et al. [21].