Figure 1. Technical aspects of common bile duct injection. a. Gross anatomy of the common bile duct and pancreatic duct in the nude mouse as seen through an upper midline laparotomy incision. b. Schematic depiction of anatomical structures seen in panel a. c. The common bile and pancreatic ducts can be imaged with autofluorescence if the anatomy is unclear. d. Injection of the common bile duct with pancreatic tumor cells using a 27 gauge needle.