Figure 2. Transcriptional activation of wild-type (WT) and P379H HNF-1alpha on the human insulin promoter in RINm5f (a.) and Hela cells (b.). Mean±standard deviation values from two independent experiments performed in sixtuplicate are indicated by the bars. Western blot analysis of wild-type and mutant HNF- 1alpha in RINm5f and HeLa cells are also shown (c.).