Figure 8. The p53 mRNA was expressed in argininetreated AR4-2J cells. a. A single band of 243 bp was seen on agarose gel from 2 to 72 hr after the addition of arginine to the culture medium. b. In the semiquantitative analysis, p53 mRNA expression was induced after 2 h, reached its peak at 24 h, then gradually decreased. Each point represents the mean±SEM of 6 observations. P values vs. previous time interval