Figure 3. TP53INP1 mRNA was expressed in arginine-treated AR4-2J cells. a. A single band of 289 bp was seen on agarose gel from 2 to 24 h after the addition of arginine into the culture medium. b. In the semiquantitative analysis, TP53INP1 mRNA was induced after 2 h, reached its peak at 6 h, then gradually decreased and disappeared at 48 hr. Each point represents the mean±SEM of 6 observations. P values vs. previous time interval.